ArticleApplied and environmental microbiology2015
Novel reporter for identification of interference with acyl homoserine lactone and autoinducer-2 quorum sensing.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- ExploringmicroLife · 2026Article
- Bacterial diseases of Asian sea bass (Heliyon · 2024Review
- Antimicrobial Peptides Originating from Expression Libraries ofMicroorganisms · 2023Article
- First insights into theFrontiers in microbiology · 2023Article
- Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Friends or Foes-Microbial Interactions in Nature.Biology · 2021Review
- Anti-quorum sensing activity of some marine bacteria isolated from different marine resources in Egypt.Biotechnology letters · 2021Article
- Cultivable microbiota associated with Aurelia aurita and Mnemiopsis leidyi.MicrobiologyOpen · 2020Article
- Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited.Scientific reports · 2020Article
- New Vocabulary for Bacterial Communication.Chembiochem : a European journal of chemical biology · 2020Review
- Agent-Based Modeling Demonstrates How Local Chemotactic Behavior Can Shape Biofilm Architecture.mSphere · 2019Article
- Widespread Existence of Quorum Sensing Inhibitors in Marine Bacteria: Potential Drugs to Combat Pathogens with Novel Strategies.Marine drugs · 2019Review
- Potential role of host-derived quorum quenching in modulating bacterial colonization in the moon jellyfish Aurelia aurita.Scientific reports · 2019Article
- Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions.Frontiers in microbiology · 2019Article
- Metagenomic quorum quenching enzymes affect biofilm formation of Candida albicans and Staphylococcus epidermidis.PloS one · 2019Article
- Review
- Article
- Evolution of the Quorum network and the mobilome (plasmids and bacteriophages) in clinical strains of Acinetobacter baumannii during a decade.Scientific reports · 2018Article
- Quorum sensing network in clinical strains of A. baumannii: AidA is a new quorum quenching enzyme.PloS one · 2017Article
- Resilience of bacterial quorum sensing against fluid flow.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two reporter strains were established to identify novel biomolecules interfering with bacterial communication (quorum sensing [QS]). The basic design of these Escherichia coli-based systems comprises a gene encoding a lethal protein fused to promoters induced in the presence of QS signal molecules. Consequently, these E. coli strains are unable to grow in the presence of the respective QS signal molecules unless a nontoxic QS-interfering compound is present. The first reporter strain designed to detect autoinducer-2 (AI-2)-interfering activities (AI2-QQ.1) contained the E. coli ccdB lethal gene under the control of the E. coli lsrA promoter. The second reporter strain (AI1-QQ.1) contained the Vibrio fischeri luxI promoter fused to the ccdB gene to detect interference with acyl-homoserine lactones. Bacteria isolated from the surfaces of several marine eukarya were screened for quorum- quenching (QQ) activities using the established reporter systems AI1-QQ.1 and AI2-QQ.1. Out of 34 isolates, two interfered with acylated homoserine lactone (AHL) signaling, five interfered with AI-2 QS signaling, and 10 were demonstrated to interfere with both signal molecules. Open reading frames (ORFs) conferring QQ activity were identified for three selected isolates (Photobacterium sp., Pseudoalteromonas sp., and Vibrio parahaemolyticus). Evaluation of the respective heterologously expressed and purified QQ proteins confirmed their ability to interfere with the AHL and AI-2 signaling processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.