ArticleBMC microbiology2014
Activation of gab cluster transcription in Bacillus thuringiensis by γ-aminobutyric acid or succinic semialdehyde is mediated by the Sigma 54-dependent transcriptional activator GabR.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- MclX modulates the process of mother cell lysis by SpoIIID inApplied and environmental microbiology · 2026Article
- Gut microbial GABA imbalance emerges as a metabolic signature in mild autism spectrum disorder linked to overrepresented Escherichia.Cell reports. Medicine · 2025Article
- Regulatory relationships amongFrontiers in microbiology · 2025Article
- Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR.Cell death discovery · 2024Article
- A Novel Regulator PepR Regulates the Expression of Dipeptidase GeneMicroorganisms · 2024Article
- Transcriptional regulation of cellobiose utilization by PRD-domain containing Sigma54-dependent transcriptional activator (CelR) and catabolite control protein A (CcpA) inFrontiers in microbiology · 2024Article
- Article
- Regulation of γ-Aminobutyrate (GABA) Utilization inFrontiers in microbiology · 2020Article
- PLP and GABA trigger GabR-mediated transcription regulation inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Disruption of Two-component System LytSR Affects Forespore Engulfment inFrontiers in cellular and infection microbiology · 2017Article
- Sox transcription in sarcosine utilization is controlled by Sigma(54) and SoxR in Bacillus thuringiensis HD73.Scientific reports · 2016Article
- Identification of metabolism pathways directly regulated by sigma(54) factor in Bacillus thuringiensis.Frontiers in microbiology · 2015Article
- Article
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8 authors.
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Abstract
backgroundBacillus thuringiensis GabR is a Sigma 54-dependent transcriptional activator containing three typical domains, an N-terminal regulatory domain Per-ARNT-Sim (PAS), a central AAA(+) (ATPases associated with different cellular activities) domain and a C-terminal helix-turn-helix (HTH) DNA binding domain. GabR positively regulates the expression of the gabT gene of the gab gene cluster, which is responsible for the γ-aminobutyric acid (GABA) shunt.
resultsPurified GabR was shown to specifically bind to a repeat region that mapped 58 bp upstream of the gabT start codon. The specific signal factors GABA and succinic semialdehyde (SSA) activated gabT expression, whereas GABA- and SSA-inducible gabT transcription was abolished in sigL and gabR mutants. GABA and SSA did not induce the expression of either SigL or GabR. Deletion of the PAS domain of GabR resulted in increased gabT transcriptional activity, both in the presence and absence of GABA.
conclusionsThis study identified the GabR-binding site on the gabT promoter; however, GabR does not bind to its own promoter. gabT transcription is induced by GABA and SSA, and inducible expression is dependent on SigL and activated by GabR. The PAS domain in GabR is repressing its enhancer transcriptional activity on the gabT promoter. Repression is released upon GABA addition, whereupon transcription is induced.
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