ArticleGenomics, proteomics & bioinformatics2014
Expression profiling and structural characterization of microRNAs in adipose tissues of hibernating ground squirrels.
Article in Genomics, proteomics & bioinformatics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Natural Killer Cell Dysfunction Is Emerging During Midlife Obesity.Aging cell · 2026Article
- Hibernation-Induced microRNA Expression Promotes Signaling Pathways and Cell Cycle Dysregulation inMetabolites · 2023Article
- Role of MicroRNAs in Extreme Animal Survival Strategies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- MicroRNA Cues from Nature: A Roadmap to Decipher and Combat Challenges in Human Health and Disease?Cells · 2021Review
- MicroRNA dynamics during hibernation of the Australian central bearded dragon (Pogona vitticeps).Scientific reports · 2020Article
- A Unique Energy-Saving Strategy during Hibernation Revealed by Multi-Omics Analysis in the Chinese Alligator.iScience · 2020Article
- Expression profiling and analysis of some miRNAs in subcutaneous white adipose tissue during development of obesity.Genes & nutrition · 2020Article
- The Torpid State: Recent Advances in Metabolic Adaptations and Protective MechanismsFrontiers in physiology · 2020Review
- Waking the sleeping dragon: gene expression profiling reveals adaptive strategies of the hibernating reptile Pogona vitticeps.BMC genomics · 2019Article
- Nature's fat-burning machine: brown adipose tissue in a hibernating mammal.The Journal of experimental biology · 2018Review
- Article
- Differential expression and emerging functions of non-coding RNAs in cold adaptation.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2017Review
- Analysis of microRNA expression during the torpor-arousal cycle of a mammalian hibernator, the 13-lined ground squirrel.Physiological genomics · 2016Article
- The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns.Scientific reports · 2016Article
- MicroRNA regulation in heart and skeletal muscle over the freeze-thaw cycle in the freeze tolerant wood frog.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Primate Torpor: Regulation of Stress-activated Protein Kinases During Daily Torpor in the Gray Mouse Lemur, Microcebus murinus.Genomics, proteomics & bioinformatics · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that are important in regulating metabolic stress. In this study, we determined the expression and structural characteristics of 20 miRNAs in brown (BAT) and white adipose tissue (WAT) during torpor in thirteen-lined ground squirrels. Using a modified stem-loop technique, we found that during torpor, expression of six miRNAs including let-7a, let-7b, miR-107, miR-150, miR-222 and miR-31 was significantly downregulated in WAT (P<0.05), which was 16%-54% of euthermic non-torpid control squirrels, whereas expression of three miRNAs including miR-143, miR-200a and miR-519d was found to be upregulated by 1.32-2.34-fold. Similarly, expression of more miRNAs was downregulated in BAT during torpor. We detected reduced expression of 6 miRNAs including miR-103a, miR-107, miR-125b, miR-21, miR-221 and miR-31 (48%-70% of control), while only expression of miR-138 was significantly upregulated (2.91±0.8-fold of the control, P<0.05). Interestingly, miRNAs found to be downregulated in WAT during torpor were similar to those dysregulated in obese humans for increased adipogenesis, whereas miRNAs with altered expression in BAT during torpor were linked to mitochondrial β-oxidation. miRPath target prediction analysis showed that miRNAs downregulated in both WAT and BAT were associated with the regulation of mitogen-activated protein kinase (MAPK) signaling, while the miRNAs upregulated in WAT were linked to transforming growth factor β (TGFβ) signaling. Compared to mouse sequences, no unique nucleotide substitutions within the stem-loop region were discovered for the associated pre-miRNAs for the miRNAs used in this study, suggesting no structure-influenced changes in pre-miRNA processing efficiency in the squirrel. As well, the expression of miRNA processing enzyme Dicer remained unchanged in both tissues during torpor. Overall, our findings suggest that changes of miRNA expression in adipose tissues may be linked to distinct biological roles in WAT and BAT during hibernation and may involve the regulation of signaling cascades.
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