Evidence map›Paper›PMID 25526634›Full record

ArticlePloS one2014

The selective antagonism of P2X7 and P2Y1 receptors prevents synaptic failure and affects cell proliferation induced by oxygen and glucose deprivation in rat dentate gyrus.

Giovanna Maraula, Daniele Lana, Elisabetta Coppi, Francesca Gentile, Tommaso Mello, Alessia Melani, Andrea Galli, Maria Grazia Giovannini, Felicita Pedata, Anna Maria Pugliese

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. AFrontiers in neuroscience · 2021
    Review
  8. AInternational journal of molecular sciences · 2020
    Review
  9. Article
  10. Article
  11. Neuronal P2X7 Receptor: Involvement in Neuronal Physiology and Pathology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Giovanna MaraulaDept. of Neuroscience, Psychology, Drug Research and Child Health, NEUROFARBA, Division of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Daniele LanaDept. of Health Sciences, Clinical Pharmacology and Oncology Unit, University of Florence, Florence, Italy.
Elisabetta CoppiDept. of Health Sciences, Clinical Pharmacology and Oncology Unit, University of Florence, Florence, Italy.
Francesca GentileDept. of Neuroscience, Psychology, Drug Research and Child Health, NEUROFARBA, Division of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Tommaso MelloDept. of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.
Alessia MelaniDept. of Neuroscience, Psychology, Drug Research and Child Health, NEUROFARBA, Division of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Andrea GalliDept. of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy.
Maria Grazia GiovanniniDept. of Health Sciences, Clinical Pharmacology and Oncology Unit, University of Florence, Florence, Italy.
Felicita PedataDept. of Neuroscience, Psychology, Drug Research and Child Health, NEUROFARBA, Division of Pharmacology and Toxicology, University of Florence, Florence, Italy.
Anna Maria PuglieseDept. of Neuroscience, Psychology, Drug Research and Child Health, NEUROFARBA, Division of Pharmacology and Toxicology, University of Florence, Florence, Italy.
University of Florence · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purinergic P2X and P2Y receptors are broadly expressed on both neurons and glial cells in the central nervous system (CNS), including dentate gyrus (DG). The aim of this research was to determine the synaptic and proliferative response of the DG to severe oxygen and glucose deprivation (OGD) in acute rat hippocampal slices and to investigate the contribution of P2X7 and P2Y1 receptor antagonism to recovery of synaptic activity after OGD. Extracellular field excitatory post-synaptic potentials (fEPSPs) in granule cells of the DG were recorded from rat hippocampal slices. Nine-min OGD elicited an irreversible loss of fEPSP and was invariably followed by the appearance of anoxic depolarization (AD). Application of MRS2179 (selective antagonist of P2Y1 receptor) and BBG (selective antagonist of P2X7 receptor), before and during OGD, prevented AD appearance and allowed a significant recovery of neurotransmission after 9-min OGD. The effects of 9-min OGD on proliferation and maturation of cells localized in the subgranular zone (SGZ) of slices prepared from rats treated with 5-Bromo-2'-deoxyuridine (BrdU) were investigated. Slices were further incubated with an immature neuron marker, doublecortin (DCX). The number of BrdU+ cells in the SGZ was significantly decreased 6 hours after OGD. This effect was antagonized by BBG, but not by MRS2179. Twenty-four hours after 9-min OGD, the number of BrdU+ cells returned to control values and a significant increase of DCX immunofluorescence was observed. This phenomenon was still evident when BBG, but not MRS2179, was applied during OGD. Furthermore, the P2Y1 antagonist reduced the number of BrdU+ cells at this time. The data demonstrate that P2X7 and P2Y1 activation contributes to early damage induced by OGD in the DG. At later stages after the insult, P2Y1 receptors might play an additional and different role in promoting cell proliferation and maturation in the DG.

Indexed as

Adenosine DiphosphateAdenosine TriphosphateAnimalsBrain IschemiaCell HypoxiaCell ProliferationCells, CulturedDentate GyrusDoublecortin ProteinGlucoseMalePurinergic P2X Receptor AntagonistsPurinergic P2Y Receptor AntagonistsRatsRats, WistarReceptors, Purinergic P2X7Adenosine DiphosphateAdenosine Triphosphatecoomassie Brilliant BlueDcx protein, ratDoublecortin ProteinGlucoseN(6)-methyl-2'-deoxyadenosine 3',5'-diphosphatePurinergic P2X Receptor AntagonistsPurinergic P2Y Receptor AntagonistsReceptors, Purinergic P2X7Receptors, Purinergic P2Y1Rosaniline Dyes

Identifiers

PMID25526634
PMCPMC4272279
OpenAlexW2089295602

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.