ArticleOrganic & biomolecular chemistry2015
Rapid and scalable assembly of firefly luciferase substrates.
Article in Organic & biomolecular chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Bioluminescence in Clinical and Point-of-Care Testing.Biosensors · 2025Review
- Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management.Journal of biological engineering · 2025Article
- A practical, biomimetic, one-pot synthesis of firefly luciferin.Scientific reports · 2024Article
- Exclusive Solvent-Controlled Regioselective Catalytic Synthesis of Potentially Bioactive Imidazolidineiminodithiones: NMR Analysis, Computational Studies and X-ray Crystal Structures.Molecules (Basel, Switzerland) · 2024Article
- Synthesis of 2-Cyanobenzothiazoles via Pd-Catalyzed/Cu-Assisted C-H Functionalization/Intramolecular C-S Bond Formation fromMolecules (Basel, Switzerland) · 2022Article
- Systematic Comparison of Beetle Luciferase-Luciferin Pairs as Sources of Near-Infrared Light for In Vitro and In Vivo Applications.International journal of molecular sciences · 2022Article
- Iodine-DMSO mediated conversion ofRSC advances · 2022Article
- Rapid Multicomponent Bioluminescence ImagingACS chemical biology · 2021Article
- Orthogonal Bioluminescent Probes from Disubstituted Luciferins.Biochemistry · 2021Article
- Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.Cell chemical biology · 2020Review
- Modified Enzyme Substrates for the Detection of Bacteria: A Review.Molecules (Basel, Switzerland) · 2020Review
- Building Biological Flashlights: Orthogonal Luciferases and Luciferins forAccounts of chemical research · 2019Review
- Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging.Chembiochem : a European journal of chemical biology · 2018Article
- Synthesis ofFrontiers in chemistry · 2018Article
- Orthogonal Luciferase-Luciferin Pairs for Bioluminescence Imaging.Journal of the American Chemical Society · 2017Article
- Brominated Luciferins Are Versatile Bioluminescent Probes.Chembiochem : a European journal of chemical biology · 2017Article
- One-pot non-enzymatic formation of firefly luciferin in a neutral buffer from p-benzoquinone and cysteine.Scientific reports · 2016Article
- Economical and scalable synthesis of 6-amino-2-cyanobenzothiazole.Beilstein journal of organic chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Bioluminescence imaging with luciferase-luciferin pairs is a popular method for visualizing biological processes in vivo. Unfortunately, most luciferins are difficult to access and remain prohibitively expensive for some imaging applications. Here we report cost-effective and efficient syntheses of d-luciferin and 6'-aminoluciferin, two widely used bioluminescent substrates. Our approach employs inexpensive anilines and Appel's salt to generate the luciferin cores in a single pot. Additionally, the syntheses are scalable and can provide multi-gram quantities of both substrates. The streamlined production and improved accessibility of luciferin reagents will bolster in vivo imaging efforts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.