ArticleThe Journal of biological chemistry2015
An atypical psbA gene encodes a sentinel D1 protein to form a physiologically relevant inactive photosystem II complex in cyanobacteria.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 37 citations in OpenAlex.
- Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon.BMC microbiology · 2025Article
- Photoacclimation strategies in cyanobacterial photosynthesis under dynamic light environments: implications in growth, fitness, and biotechnological applications.Frontiers in microbiology · 2025Review
- Exploring the Origins and Evolution of Oxygenic and Anoxygenic Photosynthesis in Deeply Branched Cyanobacteriota.Molecular biology and evolution · 2024Article
- Endogenous clock-mediated regulation of intracellular oxygen dynamics is essential for diazotrophic growth of unicellular cyanobacteria.Nature communications · 2024Article
- Transcriptome analysis of the bloom-forming dinoflagellate Prorocentrum donghaiense exposed to Ginkgo biloba leaf extract, with an emphasis on photosynthesis.Environmental science and pollution research international · 2024Article
- The endosymbiont ofISME communications · 2024Article
- Modeling the Characteristic Residues of ChlorophyllMicroorganisms · 2023Article
- Photosynthetic modulation during the diurnal cycle in a unicellular diazotrophic cyanobacterium grown under nitrogen-replete and nitrogen-fixing conditions.Scientific reports · 2022Article
- Time-resolved comparative molecular evolution of oxygenic photosynthesis.Biochimica et biophysica acta. Bioenergetics · 2021Article
- The Role of Chloroplast Gene Expression in Plant Responses to Environmental Stress.International journal of molecular sciences · 2020Review
- The diversity and distribution of D1 proteins in cyanobacteria.Photosynthesis research · 2020Article
- Chlorophyll f synthesis by a super-rogue photosystem II complex.Nature plants · 2020Article
- Functional Gene Expression in Shark Bay Hypersaline Microbial Mats: Adaptive Responses.Frontiers in microbiology · 2020Article
- Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light-dark cycle.Photosynthesis research · 2019Article
- Early Archean origin of Photosystem II.Geobiology · 2019Article
- Analysis of Protein Complexes in the Unicellular Cyanobacterium Cyanothece ATCC 51142.Journal of proteome research · 2018Article
- Reconstructing the Origin of Oxygenic Photosynthesis: Do Assembly and Photoactivation Recapitulate Evolution?Frontiers in plant science · 2016Article
- The Use of Advanced Mass Spectrometry to Dissect the Life-Cycle of Photosystem II.Frontiers in plant science · 2016Review
- Photosynthetic Versatility in the Genome ofFrontiers in microbiology · 2016Article
- Origin and Evolution of Water Oxidation before the Last Common Ancestor of the Cyanobacteria.Molecular biology and evolution · 2015Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Photosystem II, a large membrane-bound enzyme complex in cyanobacteria and chloroplasts, mediates light-induced oxidation of water to molecular oxygen. The D1 protein of PSII, encoded by the psbA gene, provides multiple ligands for cofactors crucial to this enzymatic reaction. Cyanobacteria contain multiple psbA genes that respond to various physiological cues and environmental factors. Certain unicellular cyanobacterial cells, such as Cyanothece sp. ATCC 51142, are capable of nitrogen fixation, a highly oxygen-sensitive process, by separating oxygen evolution from nitrogen fixation using a day-night cycle. We have shown that c-psbA4, one of the five psbA orthologs in this cyanobacterium, is exclusively expressed during nighttime. Remarkably, the corresponding D1 isoform has replacements of a number of amino acids that are essential ligands for the catalytic Mn4CaO5 metal center for water oxidation by PSII. At least 30 cyanobacterial strains, most of which are known to have nitrogen fixing abilities, have similar psbA orthologs. We expressed the c-psbA4 gene from Cyanothece 51142 in a 4E-3 mutant strain of the model non-nitrogen-fixing cyanobacterium Synechocystis sp. PCC 6803, which lacks any psbA gene. The resultant strain could not grow photoautotrophically. Moreover, these Synechocystis 6803 cells were incapable of PSII-mediated oxygen evolution. Based on our findings, we have named this physiologically relevant, unusual D1 isoform sentinel D1. Sentinel D1 represents a new class of D1 protein that, when incorporated in a PSII complex, ensures that PSII cannot mediate water oxidation, thus allowing oxygen-sensitive processes such as nitrogen fixation to occur in cyanobacterial cells.
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