ArticleThe Journal of biological chemistry2015
Matrix confinement plays a pivotal role in regulating neutrophil-generated tractions, speed, and integrin utilization.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- CFM: confinement force microscopy-a dynamic, precise and stable microconfiner for traction force microscopy in spatial confinement.Nature methods · 2026Article
- Mechanical communication and function regulation of immune cells.Fundamental research · 2026Review
- Monocytes use protrusive forces to generate migration paths in viscoelastic collagen-based extracellular matrices.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Generative modeling of biological shapes and images using a probabilisticbioRxiv : the preprint server for biology · 2024Article
- Transducing compressive forces into cellular outputs in cancer and beyond.Life science alliance · 2023Review
- Deformability and collision-induced reorientation enhance cell topotaxis in dense microenvironments.Biophysical journal · 2023Article
- Mechanosensitive traction force generation is regulated by the neutrophil activation state.Scientific reports · 2023Article
- Targeting Neutrophil βBiomolecules · 2023Review
- Reciprocity of Cell Mechanics with Extracellular Stimuli: Emerging Opportunities for Translational Medicine.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Regulation of biomaterial implantation-induced fibrin deposition to immunological functions of dendritic cells.Materials today. Bio · 2022Article
- Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins.Frontiers in bioengineering and biotechnology · 2022Article
- Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking.Scientific reports · 2020Article
- Bridging 2D and 3D culture: probing impact of extracellular environment on fibroblast activation in layered hydrogels.AIChE journal. American Institute of Chemical Engineers · 2019Article
- Breast Cancer Cells Transition from Mesenchymal to Amoeboid Migration in Tunable Three-Dimensional Silk-Collagen Hydrogels.ACS biomaterials science & engineering · 2019Article
- Rapid, topology-based particle tracking for high-resolution measurements of large complex 3D motion fields.Scientific reports · 2018Article
- Mechanical forces in the immune system.Nature reviews. Immunology · 2017Review
- A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix.Molecular biology of the cell · 2017Article
- Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy.Nature cell biology · 2017Article
- Leukocyte transendothelial migration: A local affair.Small GTPases · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Neutrophils are capable of switching from integrin-dependent motility on two-dimensional substrata to integrin-independent motion following entry into the confined three-dimensional matrix of an afflicted tissue. However, whether integrins still maintain a regulatory role for cell traction generation and cell locomotion under the physical confinement of the three-dimensional matrix is unknown, and this is challenging to deduce from motility studies alone. Using three-dimensional traction force microscopy and a double hydrogel sandwich system, we determined the three-dimensional spatiotemporal traction forces of motile neutrophils at unprecedented resolution and show, for the first time, that entry into a highly confined space (2.5D) is a sufficient trigger to convert to integrin-independent migration. We find that integrins exert a significant regulatory role in determining the magnitude and spatial distribution of tractions and cell speed on confined cells. We also find that 90% of neutrophil tractions are in the out-of-plane axis, and this may be a fundamental element of neutrophil traction force generation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.