ArticleScience (New York, N.Y.)2014
De novo design of a transmembrane Zn²⁺-transporting four-helix bundle.
Article in Science (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 160 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
160 citing papers in PubMed.
- Computational design of antimicrobial peptide nanopores.Nature chemical biology · 2026Article
- Design of a site selective heterochromic bimetallic lanthanide coiled coil with nanometre-scale control.Chemical science · 2026Article
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- Engineered Channel Asymmetry Extends Hydrogen-Bonding Networks for Proton Conduction.bioRxiv : the preprint server for biology · 2026Article
- Review
- Bioinspired Self-Assembly-Reinforced Ion Transport and Interface Regulation Enables Sustainable Metal-Ion Batteries for Wearable Electronics.Nano-micro letters · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Sequence Prediction, Structural Modelling and the Challenges of Plasticity in Coiled Coils.Sub-cellular biochemistry · 2026Review
- Designability of α-Helical Protein Filaments.Sub-cellular biochemistry · 2026Review
- Antibody Affinity Maturation by Computational Design.Methods in molecular biology (Clifton, N.J.) · 2026Article
- De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs.ACS nano · 2025Article
- Bottom-up design of CaNature · 2025Article
- Protein Design Meets Single-Molecule Detection: Towards Programmable Nanopore Sensors.International journal of molecular sciences · 2025Review
- Generative Landscapes and Dynamics to Design Functional Multidomain Artificial Transmembrane Transporters.ACS central science · 2025Article
- Embracing Complexity: Peptides as Tunable Scaffolds in the Construction of Discrete Supramolecular Systems.Angewandte Chemie (International ed. in English) · 2025Review
- A Designed ZnJournal of the American Chemical Society · 2025Article
- Understanding, inhibiting, and engineering membrane transporters with high-throughput mutational screens.Cell chemical biology · 2025Review
- Article
- Computational design of highly signalling-active membrane receptors through solvent-mediated allosteric networks.Nature chemistry · 2025Article
- Exploration of the hierarchical assembly space of collagen-like peptides beyond the triple helix.Nature communications · 2024Article
100 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors.
Funding
Abstract
The design of functional membrane proteins from first principles represents a grand challenge in chemistry and structural biology. Here, we report the design of a membrane-spanning, four-helical bundle that transports first-row transition metal ions Zn(2+) and Co(2+), but not Ca(2+), across membranes. The conduction path was designed to contain two di-metal binding sites that bind with negative cooperativity. X-ray crystallography and solid-state and solution nuclear magnetic resonance indicate that the overall helical bundle is formed from two tightly interacting pairs of helices, which form individual domains that interact weakly along a more dynamic interface. Vesicle flux experiments show that as Zn(2+) ions diffuse down their concentration gradients, protons are antiported. These experiments illustrate the feasibility of designing membrane proteins with predefined structural and dynamic properties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.