Evidence map›Paper›PMID 25525175›Full record

ArticleInvestigative ophthalmology & visual science2014

The use of time-lapse optical coherence tomography to image the effects of microapplied toxins on the retina.

Joseph A Majdi, Haohua Qian, Yichao Li, Robert J Langsner, Katherine I Shea, Anant Agrawal, Daniel X Hammer, Joseph P Hanig, Ethan D Cohen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Joseph A MajdiDivision of Biomedical Physics, Office of Science and Engineering Labs, Center for Devices and Radiological Health, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Haohua QianNational Eye Institute, Visual Function Core, National Institutes of Health, Bethesda, Maryland, United States.
Yichao LiNational Eye Institute, Visual Function Core, National Institutes of Health, Bethesda, Maryland, United States.
Robert J LangsnerDivision of Biomedical Physics, Office of Science and Engineering Labs, Center for Devices and Radiological Health, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Katherine I SheaOffice of Testing and Research, Center for Drug Evaluation and Research, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Anant AgrawalDivision of Biomedical Physics, Office of Science and Engineering Labs, Center for Devices and Radiological Health, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Daniel X HammerDivision of Biomedical Physics, Office of Science and Engineering Labs, Center for Devices and Radiological Health, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Joseph P HanigOffice of Testing and Research, Center for Drug Evaluation and Research, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Ethan D CohenDivision of Biomedical Physics, Office of Science and Engineering Labs, Center for Devices and Radiological Health, Food and Drug Administration, White Oak Federal Research Labs, Silver Spring, Maryland, United States.
Office of Science · USCenter for Drug Evaluation and Research · USCenter for Devices and Radiological Health · USNational Eye Institute · USNational Institutes of Health · US

Funding

Evaluation of Visual Function in Animal ModelsZIAEY000530 · NEI · NATIONAL EYE INSTITUTE · PI QIAN, HAOHUA · 2013 to 2025
$1.8M
6 · The paper itself

Abstract

purposeWe developed a novel technique for accelerated drug screening and retinotoxin characterization using time-lapse optical coherence tomography (OCT) and a drug microapplication device.

methodsUsing an ex vivo rabbit eyecup preparation, we studied retinotoxin effects in real-time by microperfusing small retinal areas under a transparent fluoropolymer tube. Known retinotoxic agents were applied to the retina for 5-minute periods, while changes in retinal structure, thickness, and reflectance were monitored with OCT. The OCT images of two agents with dissimilar mechanisms, cyanide and kainic acid, were compared to their structural changes seen histologically.

resultsWe found the actions of retinotoxic agents tested could be classified broadly into two distinct types: (1) agents that induce neuronal depolarization, such as kainic acid, causing increases in OCT reflectivity or thickness of the inner plexiform and nuclear layers, and decreased reflectivity of the outer retina; and (2) agents that disrupt mitochondrial function, such as cyanide, causing outer retinal structural changes as evidenced by a reduction in the OCT reflectivity of the photoreceptor outer segment and pigment epithelium layers.

conclusionsRetinotoxin-induced changes in retinal layer reflectivity and thickness under the microperfusion tube in OCT images closely matched the histological evidence of retinal injury. Time-lapse OCT imaging of the microperfused local retina has the potential to accelerate drug retinotoxicological screening and expand the use of OCT as an evaluation tool for preclinical animal testing.

Indexed as

AnimalsDisease Models, AnimalDose-Response Relationship, DrugFemaleFollow-Up StudiesKainic AcidMaleRabbitsRetinal DiseasesRetinal Photoreceptor Cell Outer SegmentRetinal Pigment EpitheliumTime FactorsTomography, Optical CoherenceKainic Acidmicroperfusionoptical coherence tomographyretinotoxins

Identifiers

PMID25525175
PMCPMC4544596
OpenAlexW2019811516

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.