ArticlePlant physiology2015
PECTIN METHYLESTERASE48 is involved in Arabidopsis pollen grain germination.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 125 citations in OpenAlex.
- Pectin Structural Dynamics: Developmental and Evolutionary Perspectives.Plants (Basel, Switzerland) · 2026Review
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction.Plant reproduction · 2026Review
- Plant movements: navigating the light environment.Plant physiology · 2026Review
- Advances in the functional research of pectin methylesterases in Arabidopsis thaliana.BMC plant biology · 2026Review
- Resolving sampling and population-size biases in domestication genomics supports a South Asian origin of walnuts.Genome biology · 2026Article
- Hidden Xyloglucan Architecture of the Pollen Intine inBiology · 2026Article
- Mechanosensitive ion channel MSL8 is required for oscillatory growth and cell wall dynamics in Arabidopsis pollen tubes.Plant reproduction · 2025Article
- Pectin Metabolism Influences Phloem Architecture and Flowering Time in Arabidopsis Thaliana.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Characterization and Transcriptome Analysis Reveal Abnormal Pollen Germination in Cytoplasmic Male Sterile Tomato.International journal of molecular sciences · 2025Article
- Analysis of Meiotic Behavior and 2n Pollen Formation Frequency in Triploid Hybrids of Chinese Jujube.Plants (Basel, Switzerland) · 2025Article
- Article
- Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility.Plant communications · 2024Review
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Gene Regulatory Network Controlling Flower Development in Spinach (International journal of molecular sciences · 2024Article
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Pectin methylesterification state and cell wall mechanical properties contribute to neighbor proximity-induced hypocotyl growth in Arabidopsis.Plant direct · 2024Article
- Stop and go signals at the stigma-pollen interface of the Brassicaceae.Plant physiology · 2023Article
- The hydroxyproline O-arabinosyltransferase FIN4 is required for tomato pollen intine development.Plant reproduction · 2023Article
- The cotton pectin methyl esterase gene GhPME21 functions in microspore development and fertility in Gossypium hirsutum L.Plant molecular biology · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germination of pollen grains is a crucial step in plant reproduction. However, the molecular mechanisms involved remain unclear. We investigated the role of PECTIN METHYLESTERASE48 (PME48), an enzyme implicated in the remodeling of pectins in Arabidopsis (Arabidopsis thaliana) pollen. A combination of functional genomics, gene expression, in vivo and in vitro pollen germination, immunolabeling, and biochemical analyses was used on wild-type and Atpme48 mutant plants. We showed that AtPME48 is specifically expressed in the male gametophyte and is the second most expressed PME in dry and imbibed pollen grains. Pollen grains from homozygous mutant lines displayed a significant delay in imbibition and germination in vitro and in vivo. Moreover, numerous pollen grains showed two tips emerging instead of one in the wild type. Immunolabeling and Fourier transform infrared analyses showed that the degree of methylesterification of the homogalacturonan was higher in pme48-/- pollen grains. In contrast, the PME activity was lower in pme48-/-, partly due to a reduction of PME48 activity revealed by zymogram. Interestingly, the wild-type phenotype was restored in pme48-/- with the optimum germination medium supplemented with 2.5 mm calcium chloride, suggesting that in the wild-type pollen, the weakly methylesterified homogalacturonan is a source of Ca(2+) necessary for pollen germination. Although pollen-specific PMEs are traditionally associated with pollen tube elongation, this study provides strong evidence that PME48 impacts the mechanical properties of the intine wall during maturation of the pollen grain, which, in turn, influences pollen grain germination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.