ArticleStem cells (Dayton, Ohio)2015
Isolation and characterization of embryonic stem cell-derived cardiac Purkinje cells.
Article in Stem cells (Dayton, Ohio), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Towards chamber specific heart-on-a-chip for drug testing applications.Advanced drug delivery reviews · 2020Pooled it
- Transient Notch Activation Converts Pluripotent Stem Cell-Derived Cardiomyocytes Towards a Purkinje Fiber Fate.bioRxiv : the preprint server for biology · 2024Article
- Cardiac conduction diseases: understanding the molecular mechanisms to uncover targets for future treatments.Expert opinion on therapeutic targets · 2024Review
- Heart rhythmFrontiers in cardiovascular medicine · 2024Article
- The transcription factor EBF1 non-cell-autonomously regulates cardiac growth and differentiation.Development (Cambridge, England) · 2023Article
- Knockout of the Cardiac Transcription Factor NKX2-5 Results in Stem Cell-Derived Cardiac Cells with Typical Purkinje Cell-like Signal Transduction and Extracellular Matrix Formation.International journal of molecular sciences · 2023Article
- Application of Three-Dimensional Culture Method in the Cardiac Conduction System Research.Methods and protocols · 2022Article
- A novel transgenic Cre allele to label mouse cardiac conduction system.Developmental biology · 2021Article
- Neural cell adhesion molecule is required for ventricular conduction system development.Development (Cambridge, England) · 2021Article
- Enhancing Matured Stem-Cardiac Cell Generation and Transplantation: A Novel Strategy for Heart Failure Therapy.Journal of cardiovascular translational research · 2021Review
- Response to Zhao and Huang's Commentary Letter, "Conversion of Human Cardiac Progenitor Cells using Reprogramming Factors into Heterogeneous Cardiac Pacemaker-like Cells", regarding our Manuscript: "Conversion of Human Cardiac Progenitor Cells into Cardiac Pacemaker-like Cells".Journal of molecular and cellular cardiology · 2020Article
- 3D bioprinting and its potential impact on cardiac failure treatment: An industry perspective.APL bioengineering · 2020Article
- A human embryonic stem cell reporter line for monitoring chemical-induced cardiotoxicity.Cardiovascular research · 2020Article
- Conversion of human cardiac progenitor cells into cardiac pacemaker-like cells.Journal of molecular and cellular cardiology · 2020Article
- TRANSCRIPTIONAL REGULATION OF THE CARDIAC CONDUCTION SYSTEM.Transactions of the American Clinical and Climatological Association · 2020Article
- Fates Aligned: Origins and Mechanisms of Ventricular Conduction System and Ventricular Wall Development.Pediatric cardiology · 2018Review
- ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes.Scientific reports · 2018Article
- Big bottlenecks in cardiovascular tissue engineering.Communications biology · 2018Article
- Probing early heart development to instruct stem cell differentiation strategies.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Review
- Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes.Nature communications · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The cardiac Purkinje fiber network is composed of highly specialized cardiomyocytes responsible for the synchronous excitation and contraction of the ventricles. Computational modeling, experimental animal studies, and intracardiac electrical recordings from patients with heritable and acquired forms of heart disease suggest that Purkinje cells (PCs) may also serve as critical triggers of life-threatening arrhythmias. Nonetheless, owing to the difficulty in isolating and studying this rare population of cells, the precise role of PC in arrhythmogenesis and the underlying molecular mechanisms responsible for their proarrhythmic behavior are not fully characterized. Conceptually, a stem cell-based model system might facilitate studies of PC-dependent arrhythmia mechanisms and serve as a platform to test novel therapeutics. Here, we describe the generation of murine embryonic stem cells (ESC) harboring pan-cardiomyocyte and PC-specific reporter genes. We demonstrate that the dual reporter gene strategy may be used to identify and isolate the rare ESC-derived PC (ESC-PC) from a mixed population of cardiogenic cells. ESC-PC display transcriptional signatures and functional properties, including action potentials, intracellular calcium cycling, and chronotropic behavior comparable to endogenous PC. Our results suggest that stem-cell derived PC are a feasible new platform for studies of developmental biology, disease pathogenesis, and screening for novel antiarrhythmic therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.