ArticleThe Journal of physiology2015
Postsynaptic P2X3-containing receptors in gustatory nerve fibres mediate responses to all taste qualities in mice.
Article in The Journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 1 of them a synthesis that pooled it.
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Who cites it
60 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Efficacy and safety of gefapixant for chronic cough: a meta-analysis of randomised controlled trials.European respiratory review : an official journal of the European Respiratory Society · 2023Pooled it
- The P2X3 receptor antagonist filapixant in patients with refractory chronic cough: a randomized controlled trial.Respiratory research · 2023Trial
- Safety, Pharmacodynamics, and Pharmacokinetics of P2X3 Receptor Antagonist Eliapixant (BAY 1817080) in Healthy Subjects: Double-Blind Randomized Study.Clinical pharmacokinetics · 2022Trial
- Randomised trial of the P2XThe European respiratory journal · 2022Trial
- The structure and function of taste G protein-coupled receptors and their implications in diseases.International journal of oral science · 2026Review
- Neuropeptide Y and peptide YY differentially modulate bitter- and fatty acid-evoked responses in human fungiform taste bud cells.American journal of physiology. Cell physiology · 2026Article
- The New Wave of Blocking Taste.The journal of pediatric pharmacology and therapeutics : JPPT : the official journal of PPAG · 2025Article
- Safety, Tolerability, and Pharmacokinetics of Filapixant, a Highly Selective P2X3 Receptor Antagonist, in an Ascending-Single-Dose First-in-Human Study.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Bitter and Sweet Diets Alter Taste Response and Alcohol Consumption Behavior in Mice.Nutrients · 2025Article
- Article
- Increased Purinergic Signaling in Human Dental Pulps With Inflammatory Pain is Sex-Dependent.The journal of pain · 2024Article
- Patient preferences for the treatment of chronic cough: a discrete choice experiment.BMJ open respiratory research · 2024Article
- Give-and-take of gustation: the interplay between gustatory neurons and taste buds.Chemical senses · 2024Review
- DT-0111: a novel P2X3 receptor antagonist.Purinergic signalling · 2023Article
- Article
- Pungency Perception and the Interaction with Basic Taste Sensations: An Overview.Foods (Basel, Switzerland) · 2023Review
- Efficacy and Safety of Gefapixant for Refractory or Unexplained Chronic Cough over 52 Weeks.American journal of respiratory and critical care medicine · 2023Article
- The elusive cephalic phase insulin response: triggers, mechanisms, and functions.Physiological reviews · 2023Review
- Variability in P2X receptor composition in human taste nerves: implications for treatment of chronic cough.ERJ open research · 2023Article
- The Role of ATP and Purinergic Receptors in Taste Signaling.Handbook of experimental pharmacology · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Taste buds release ATP to activate ionotropic purinoceptors composed of P2X2 and P2X3 subunits, present on the taste nerves. Mice with genetic deletion of P2X2 and P2X3 receptors (double knockout mice) lack responses to all taste stimuli presumably due to the absence of ATP-gated receptors on the afferent nerves. Recent experiments on the double knockout mice showed, however, that their taste buds fail to release ATP, suggesting the possibility of pleiotropic deficits in these global knockouts. To test further the role of postsynaptic P2X receptors in afferent signalling, we used AF-353, a selective antagonist of P2X3-containing receptors to inhibit the receptors acutely during taste nerve recording and behaviour. The specificity of AF-353 for P2X3-containing receptors was tested by recording Ca(2+) transients to exogenously applied ATP in fura-2 loaded isolated geniculate ganglion neurons from wild-type and P2X3 knockout mice. ATP responses were completely inhibited by 10 μm or 100 μm AF-353, but neither concentration blocked responses in P2X3 single knockout mice wherein the ganglion cells express only P2X2-containing receptors. Furthermore, AF-353 had no effect on taste-evoked ATP release from taste buds. In wild-type mice, i.p. injection of AF-353 or simple application of the drug directly to the tongue, inhibited taste nerve responses to all taste qualities in a dose-dependent fashion. A brief access behavioural assay confirmed the electrophysiological results and showed that preference for a synthetic sweetener, SC-45647, was abolished following i.p. injection of AF-353. These data indicate that activation of P2X3-containing receptors is required for transmission of all taste qualities.
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