ArticlePflugers Archiv : European journal of physiology1989
Calcium channel current and its sensitivity to (+) isradipine in cultured pregnant rat myometrial cells. An electrophysiological and a binding study.
Article in Pflugers Archiv : European journal of physiology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Role of T-type Ca Channels in the Spontaneous Phasic Contraction of Pregnant Rat Uterine Smooth Muscle.The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology · 2009Article
- Properties of voltage-activated [Ca2+]i transients in single smooth muscle cells isolated from pregnant rat uterus.The Journal of physiology · 1998Article
- Effects of cyclopiazonic acid on rhythmic contractions in uterine smooth muscle bundles of the rat.British journal of pharmacology · 1994Article
- The action of amlodipine on voltage-operated calcium channels in vascular smooth muscle.British journal of pharmacology · 1993Article
- Effect of dihydropyridines on calcium channels in isolated smooth muscle cells from rat vena cava.British journal of pharmacology · 1992Article
- Electrophysiological properties of membrane currents in single myometrial cells isolated from pregnant rats.Pflugers Archiv : European journal of physiology · 1991Article
- Fenoverine inhibition of calcium channel currents in single smooth muscle cells from rat portal vein and myometrium.British journal of pharmacology · 1991Article
- Effects of 2,3-butanedione monoxime on whole-cell Ca2+ channel currents in single cells of the guinea-pig taenia caeci.The Journal of physiology · 1991Article
- Calcium currents in the A7r5 smooth muscle-derived cell line.Pflugers Archiv : European journal of physiology · 1990Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Action of (+) isradipine (PN 200-110), a dihydropyridine derivative, was investigated on the Ca channel current in cultured cells obtained from the longitudinal layer of the pregnant rat myometrium (18-19 days of gestation). Under our experimental conditions, the inward current was attributed to L-type inward current since: (i) equimolar replacement of Ba for Ca induced an increase in the peak current and a decrease in inactivation rate; (ii) residual inward currents were recorded at the end of the pulse; (iii) membrane potential for mid inactivation was about -40 mV; (iv) the voltage dependencies of the peak current elicited from holding potentials of -40 mV and -80 mV were similar. The inward current could be reduced with nanomolar concentrations of (+) isradipine when cells were depolarized by pulses to positive potentials. This was characterized by a pronounced initial blockade, but by no increased in blockade when pulses were repeatedly applied at a frequency of 0.05 Hz. Using the double pulse procedure we confirmed that (+) isradipine did not bind to the open-state of the Ca channels. Voltage-dependence of (+) isradipine blockade was assessed by determining the steady-state availability of the Ca channels. From the shift of the inactivation curve in the presence of (+) isradipine we calculated a (K)I value of 130 pM. Scatchard analysis of the specific binding of (+)[3H] isradipine resulted in a linear plot, thereby indicating specific binding to a single class of sites with a dissociation constant Kd of about 100 pM.(ABSTRACT TRUNCATED AT 250 WORDS)
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