Evidence map›Paper›PMID 25522967›Full record

ArticleBiomaterials2015

Immunomodulation by mesenchymal stem cells combats the foreign body response to cell-laden synthetic hydrogels.

Mark D Swartzlander, Anna K Blakney, Luke D Amer, Kurt D Hankenson, Themis R Kyriakides, Stephanie J Bryant

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
5.6field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 132 citations in OpenAlex.

  1. Article
  2. Review
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  5. Article
  6. Review
  7. Review
  8. Polylactide Degradation Activates Immune Cells by Metabolic Reprogramming.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Fast-Curing Injectable Microporous Hydrogel forACS applied bio materials · 2022
    Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Mark D SwartzlanderDepartment of Chemical & Biological Engineering, University of Colorado, Boulder, CO 80309, USA; Biofrontiers Institute, University of Colorado, Boulder, CO 80309, USA. Electronic address: Mark.Swartzlander@colorado.edu.
Anna K BlakneyDepartment of Chemical & Biological Engineering, University of Colorado, Boulder, CO 80309, USA. Electronic address: Blakney@uw.edu.
Luke D AmerDepartment of Chemical & Biological Engineering, University of Colorado, Boulder, CO 80309, USA; Biofrontiers Institute, University of Colorado, Boulder, CO 80309, USA. Electronic address: Luke.Amer@colorado.edu.
Kurt D HankensonDepartment of Small Animal Clinical Science, School of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA; Department of Physiology, Colleges of Natural Sciences and Osteopathic Medicine, Michigan State University, East Lansing, MI 48824, USA. Electronic address: kdhank@msu.edu.
Themis R KyriakidesDepartment of Pathology, Yale University School of Medicine, New Haven, CT 06509, USA. Electronic address: Themis.Kyriakides@yale.edu.
Stephanie J BryantDepartment of Chemical & Biological Engineering, University of Colorado, Boulder, CO 80309, USA; Biofrontiers Institute, University of Colorado, Boulder, CO 80309, USA; Material Science and Engineering Program, University of Colorado, Boulder, CO 80309, USA. Electronic address: Stephanie.Bryant@colorado.edu.
University of Colorado Boulder · USMichigan State University · USYale University · US

Funding

PREPARATION AND DISTRUBUTION OF ADULT STEM CELLS: HUMAN &RODENTP40RR017447 · NCRR · TULANE UNIVERSITY OF LOUISIANA · PI PROCKOP, DARWIN JOHNSON · 2003 to 2011
$8.9M
Mechanical Stimulation of Cells in Photopolymerized GelsK22DE016608 · NIDCR · UNIVERSITY OF COLORADO AT BOULDER · PI BRYANT, STEPHANIE J · 2005 to 2009
$552k
The Interplay between Macrophages and Differentiating MSCs in Cell-Laden HydrogelR21AR064436 · NIAMS · UNIVERSITY OF COLORADO · PI BRYANT, STEPHANIE J · 2013 to 2014
$358k
Modulating the Host Response to Tissue Engineering ScaffoldsR03DE019505 · NIDCR · UNIVERSITY OF COLORADO · PI BRYANT, STEPHANIE J · 2010 to 2011
$231k
NCRR NIH HHS P40 RR017447NCRR NIH HHS P40RR017447NIAMS NIH HHS 1R21AR064436NIAMS NIH HHS R21 AR064436NIDCR NIH HHS 1R03DE019505NIDCR NIH HHS K22 DE016608NIDCR NIH HHS K22DE016608NIDCR NIH HHS R03 DE019505
6 · The paper itself

Abstract

The implantation of non-biological materials, including scaffolds for tissue engineering, ubiquitously leads to a foreign body response (FBR). We recently reported that this response negatively impacts fibroblasts encapsulated within a synthetic hydrogel and in turn leads to a more severe FBR, suggesting a cross-talk between encapsulated cells and inflammatory cells. Given the promise of mesenchymal stem cells (MSCs) in tissue engineering and recent evidence of their immunomodulatory properties, we hypothesized that MSCs encapsulated within poly(ethylene glycol) (PEG) hydrogels will attenuate the FBR. In vitro, murine MSCs encapsulated within PEG hydrogels attenuated classically activated primary murine macrophages by reducing gene expression and protein secretion of pro-inflammatory cytokines, most notably tumor necrosis factor-α. Using a COX2 inhibitor, prostaglandin E2 (PGE2) was identified as a mediator of MSC immunomodulation of macrophages. In vivo, hydrogels laden with MSCs, osteogenically differentiating MSCs, or no cells were implanted subcutaneously into C57BL/6 mice for 28 days to assess the impact of MSCs on the fibrotic response of the FBR. The presence of encapsulated MSCs reduced fibrous capsule thickness compared to acellular hydrogels, but this effect diminished with osteogenic differentiation. The use of MSCs prior to differentiation in tissue engineering may therefore serve as a dynamic approach, through continuous cross-talk between MSCs and the inflammatory cells, to modulate macrophage activation and attenuate the FBR to implanted synthetic scaffolds thus improving the long-term tissue engineering outcome.

Indexed as

AnimalsCell DifferentiationCells, ImmobilizedDinoprostoneForeign-Body ReactionHydrogel, Polyethylene Glycol DimethacrylateImmunomodulationIntracellular SpaceLipopolysaccharidesMacrophage ActivationMaleMesenchymal Stem CellsMice, Inbred C57BLOsteogenesisParacrine CommunicationDinoprostoneHydrogel, Polyethylene Glycol DimethacrylateLipopolysaccharidesForeign body responseHydrogelImmunomodulationMacrophageMesenchymal stem cellPoly(ethylene glycol)

Identifiers

PMID25522967
PMCPMC5577802
OpenAlexW2031991121

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.