ArticleNano letters2015
Two color DNA barcode detection in photoluminescence suppressed silicon nitride nanopores.
Article in Nano letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Interrogating nanopores with light: optipore sensing for single molecule analyses.Journal of nanobiotechnology · 2026Review
- Nanomechanical thermometry for probing sub-nW thermal transport.Microsystems & nanoengineering · 2024Article
- Label-Free Imaging of DNA Interactions with 2D Materials.ACS photonics · 2024Article
- Single-molecule fluorescence methods for protein biomarker analysis.Analytical and bioanalytical chemistry · 2023Review
- Localized Nanopore Fabrication via Controlled Breakdown.Nanomaterials (Basel, Switzerland) · 2022Review
- Enhanced Optical Spectroscopy for Multiplexed DNA and Protein-Sequencing with Plasmonic Nanopores: Challenges and Prospects.Analytical chemistry · 2022Article
- Recent advances in integrated solid-state nanopore sensors.Lab on a chip · 2021Review
- Nanopore Identification of Single Nucleotide Mutations in Circulating Tumor DNA by Multiplexed Ligation.Clinical chemistry · 2021Article
- Dynamics of DNA Clogging in Hafnium Oxide Nanopores.The journal of physical chemistry. B · 2020Article
- Engineering improved measurement and actuation for nanoscale biophysics.Biophysical reviews · 2020Article
- Microfluidic Systems Applied in Solid-State Nanopore Sensors.Micromachines · 2020Review
- Simulation of single-protein nanopore sensing shows feasibility for whole-proteome identification.PLoS computational biology · 2019Article
- Optically-Monitored Nanopore Fabrication Using a Focused Laser Beam.Scientific reports · 2018Article
- Solid-State Nanopore.Nanoscale research letters · 2018Review
- Fabrication of multiple nanopores in a SiNScientific reports · 2018Article
- Length-independent DNA packing into nanopore zero-mode waveguides for low-input DNA sequencing.Nature nanotechnology · 2017Article
- Optofluidic devices with integrated solid-state nanopores.Mikrochimica acta · 2016Article
- Direct Sensing and Discrimination among Ubiquitin and Ubiquitin Chains Using Solid-State Nanopores.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Optical sensing of solid-state nanopores is a relatively new approach that can enable high-throughput, multicolor readout from a collection of nanopores. It is therefore highly attractive for applications such as nanopore-based DNA sequencing and genotyping using DNA barcodes. However, to date optical readout has been plagued by the need to achieve sufficiently high signal-to-noise ratio (SNR) for single fluorophore sensing, while still maintaining millisecond resolution. One of the main factors degrading the optical SNR in solid-state nanopores is the high photoluminescence (PL) background emanating from the silicon nitride (SiNx) membrane in which pores are commonly fabricated. Focusing on the optical properties of SiNx nanopores we show that the local membrane PL intensity is substantially reduced, and its spectrum is shifted toward shorter wavelengths with increasing e-beam dose. This phenomenon, which is correlated with a marked photocurrent enhancement in these nanopores, is utilized to perform for the first time single molecule fluorescence detection using both green and red laser excitations. Specifically, the reduction in PL and the concurrent measurement of the nanopore photocurrent enhancement allow us to maximize the background suppression and to detect a dual color, five-unit DNA barcode with high SNR levels.
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