Evidence map›Paper›PMID 25522428›Full record

ArticleThe international journal of neuropsychopharmacology2014

Behavioral and neurochemical changes in mesostriatal dopaminergic regions of the rat after chronic administration of the cannabinoid receptor agonist WIN55,212-2.

Eleni Fanarioti, Maria Mavrikaki, George Panagis, Ada Mitsacos, George G Nomikos, Panagiotis Giompres

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The international journal of neuropsychopharmacology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Eleni FanariotiUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos).
Maria MavrikakiUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos).
George PanagisUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos).
Ada MitsacosUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos).
George G NomikosUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos).
Panagiotis GiompresUniversity of Patras, Department of Biology, Laboratory of Human and Animal Physiology, Patras, Greece (Drs Fanarioti and Giompres); University of Crete, Department of Psychology, Laboratory of Behavioral Neuroscience, Rethymno, Crete, Greece (Drs Mavrikaki and Panagis); University of Patras, Department of Medicine, Laboratory of Physiology, Patras (Dr Mitsacos); Takeda Development Center Americas Inc., Deerfield, IL (Dr Nomikos). giompres@upatras.gr.
University of Crete · GRCrete University Press · GRUniversity of Patras · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe endocannabinoid system interacts extensively with other neurotransmitter systems and has been implicated in a variety of functions, including regulation of basal ganglia circuits and motor behavior. The present study examined the effects of repeated administration of the nonselective cannabinoid receptor 1 agonist WIN55,212-2 on locomotor activity and on binding and mRNA levels of dopamine receptors and transporters and GABAA receptors in mesostriatal dopaminergic regions of the rat.

methodsRats received systemic injections of WIN55,212-2 (0, 0.1, 0.3, or 1mg/kg, intraperitoneally) for 20 consecutive days. Locomotor activity was measured on days 1, 10, and 20. Following the last measurement, rats were euthanized and prepared for in vitro binding and in situ hybridization experiments.

resultsAcutely, 0.3 and 1mg/kg of WIN55,212-2 produced hypolocomotion, which was sustained for the next 2 measurements, compared to vehicle. Repeated administration of WIN55,212-2 decreased the mRNA levels of the D2 autoreceptors in substantia nigra and ventral tegmental area and increased D1 receptor mRNA and binding in nucleus accumbens. Furthermore, both dopamine receptor and transporter binding and mRNA levels were decreased in substantia nigra. Moreover, repeated administration of WIN55,212-2 decreased GABAA receptor binding levels in dorsal striatum and substantia nigra.

conclusionsOur data indicate that chronic WIN55,212-2 administration results in sustained effects on locomotor activity, similar to those observed after acute administration, and modulates the dopaminergic and GABAergic systems in a region-, dose-, and neurotransmitter-selective manner.

Indexed as

AnimalsBasal GangliaBehavior, AnimalBenzoxazinesCannabinoid Receptor AgonistsDopamine Plasma Membrane Transport ProteinsDopaminergic NeuronsDose-Response Relationship, DrugInjections, IntraperitonealMaleMorpholinesMotor ActivityNaphthalenesRats, Sprague-DawleyReceptor, Cannabinoid, CB1Receptors, Dopamine D1(3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanoneBenzoxazinesCannabinoid Receptor AgonistsCnr1 protein, ratDopamine Plasma Membrane Transport ProteinsMorpholinesNaphthalenesReceptor, Cannabinoid, CB1Receptors, Dopamine D1Receptors, Dopamine D2Receptors, GABA-ARNA, Messenger212-2dopamine receptorsdopamine transporterGABAA receptorlocomotor activityWIN55

Identifiers

PMID25522428
PMCPMC4438542
OpenAlexW2132479433

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.