ArticleMagnetic resonance in medicine2015
Engineering an effective Mn-binding MRI reporter protein by subcellular targeting.
Article in Magnetic resonance in medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Genetic control of MRI contrast using the manganese transporter Zip14.Magnetic resonance in medicine · 2024Article
- A Dual-Gene Reporter-Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange.Chembiochem : a European journal of chemical biology · 2024Article
- A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange.bioRxiv : the preprint server for biology · 2024Article
- Designing Protein-Based Probes for Sensing Biological Analytes with Magnetic Resonance Imaging.Analysis & sensing · 2022Article
- Review
- Article
- Beyond the Green Fluorescent Protein: Biomolecular Reporters for Anaerobic and Deep-Tissue Imaging.Bioconjugate chemistry · 2020Review
- Cell Tracking in Cancer Immunotherapy.Frontiers in medicine · 2020Review
- Manganese-Enhanced Magnetic Resonance Imaging: Overview and Central Nervous System Applications With a Focus on Neurodegeneration.Frontiers in aging neuroscience · 2018Review
- Biomolecular MRI reporters: Evolution of new mechanisms.Progress in nuclear magnetic resonance spectroscopy · 2017Review
- NMR Hyperpolarization Techniques of Gases.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Review
- Non-invasive imaging using reporter genes altering cellular water permeability.Nature communications · 2016Article
- Co-Registration of Bioluminescence Tomography, Computed Tomography, and Magnetic Resonance Imaging for MultimodalTomography (Ann Arbor, Mich.) · 2016Article
- High magnetic field induced otolith fusion in the zebrafish larvae.Scientific reports · 2016Article
- Development of Timd2 as a reporter gene for MRI.Magnetic resonance in medicine · 2016Article
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Authors and funding
5 authors.
Funding
Abstract
purposeManganese (Mn) is an effective contrast agent and biologically active metal, which has been widely used for Mn-enhanced MRI (MEMRI). The purpose of this study was to develop and test a Mn binding protein for use as a genetic reporter for MEMRI.
methodsThe bacterial Mn-binding protein, MntR was identified as a candidate reporter protein. MntR was engineered for expression in mammalian cells, and targeted to different subcellular organelles, including the Golgi Apparatus where cellular Mn is enriched. Transfected HEK293 cells and B16 melanoma cells were tested in vitro and in vivo, using immunocytochemistry, MR imaging and relaxometry.
resultsSubcellular targeting of MntR to the cytosol, endoplasmic reticulum and Golgi apparatus was verified with immunocytochemistry. After targeting to the Golgi, MntR expression produced robust R1 changes and T1 contrast in cells, in vitro and in vivo. Co-expression with the divalent metal transporter DMT1, a previously described Mn-based reporter, further enhanced contrast in B16 cells in culture, but in the in vivo B16 tumor model tested was not significantly better than MntR alone.
conclusionThis second-generation reporter system both expands the capabilities of genetically encoded reporters for imaging with MEMRI and provides important insights into the mechanisms of Mn biology which create endogenous MEMRI contrast.
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