ArticlePloS one2014
Cardiogel: a nano-matrix scaffold with potential application in cardiac regeneration using mesenchymal stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Review
- BMSCs and Osteoblast-Engineered ECM Synergetically Promotes Osteogenesis and Angiogenesis in an Ectopic Bone Formation Model.Frontiers in bioengineering and biotechnology · 2022Article
- Mesenchymal Stem Cells for Cardiac Regeneration: from Differentiation to Cell Delivery.Stem cell reviews and reports · 2021Review
- Milestones and current achievements in development of multifunctional bioscaffolds for medical application.Bioactive materials · 2021Review
- Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem-Cell-Derived ECM for Cartilage Tissue Engineering.Advanced healthcare materials · 2021Article
- Low intermittent flow promotes rat mesenchymal stem cell differentiation in logarithmic fluid shear device.Biomicrofluidics · 2020Article
- Natural Biomaterials for Cardiac Tissue Engineering: A Highly Biocompatible Solution.Frontiers in cardiovascular medicine · 2020Review
- Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes.International journal of molecular sciences · 2019Review
- Review
- Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in vitro.Molecular medicine reports · 2017Article
- New Delivery Systems of Stem Cells for Vascular Regeneration in Ischemia.Frontiers in cardiovascular medicine · 2017Review
- Artificial Cardiac Muscle with or without the Use of Scaffolds.BioMed research international · 2017Review
- Mesenchymal stem cells in cardiac regeneration: a detailed progress report of the last 6 years (2010-2015).Stem cell research & therapy · 2016Review
- Clinical efficacy and safety of autologous stem cell transplantation for patients with ST-segment elevation myocardial infarction.Therapeutics and clinical risk management · 2016Article
- The Clinical Status of Stem Cell Therapy for Ischemic Cardiomyopathy.Stem cells international · 2015Review
- Cardiac Extracellular Matrix Modification as a Therapeutic Approach.Advances in experimental medicine and biologyReview
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3-Dimensional conditions for the culture of Bone Marrow-derived Stromal/Stem Cells (BMSCs) can be generated with scaffolds of biological origin. Cardiogel, a cardiac fibroblast-derived Extracellular Matrix (ECM) has been previously shown to promote cardiomyogenic differentiation of BMSCs and provide protection against oxidative stress. To determine the matrix composition and identify significant proteins in cardiogel, we investigated the differences in the composition of this nanomatrix and a BMSC-derived ECM scaffold, termed as 'mesogel'. An optimized protocol was developed that resulted in efficient decellularization while providing the maximum yield of ECM. The proteins were sequentially solubilized using acetic acid, Sodium Dodecyl Sulfate (SDS) and Dithiothreitol (DTT). These proteins were then analyzed using surfactant-assisted in-solution digestion followed by nano-liquid chromatography and tandem mass spectrometry (nLC-MS/MS). The results of these analyses revealed significant differences in their respective compositions and 17 significant ECM/matricellular proteins were differentially identified between cardiogel and mesogel. We observed that cardiogel also promoted cell proliferation, adhesion and migration while enhancing cardiomyogenic differentiation and angiogenesis. In conclusion, we developed a reproducible method for efficient extraction and solubilization of in vitro cultured cell-derived extracellular matrix. We report several important proteins differentially identified between cardiogel and mesogel, which can explain the biological properties of cardiogel. We also demonstrated the cardiomyogenic differentiation and angiogenic potential of cardiogel even in the absence of any external growth factors. The transplantation of Bone Marrow derived Stromal/Stem Cells (BMSCs) cultured on such a nanomatrix has potential applications in regenerative therapy for Myocardial Infarction (MI).
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