ArticleChembiochem : a European journal of chemical biology2015
Hen egg-white lysozyme crystallisation: protein stacking and structure stability enhanced by a Tellurium(VI)-centred polyoxotungstate.
Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Article
- Speciation atlas of polyoxometalates in aqueous solutions.Science advances · 2023Article
- Synthesis and characterization of the `Japanese rice-ball'-shaped Molybdenum Blue NaActa crystallographica. Section C, Structural chemistry · 2022Article
- Conformational flexibility in the zinc solute-binding protein ZnuA.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Speciation of Transition-Metal-Substituted Keggin-Type Silicotungstates Affected by the Co-crystallization Conditions with Proteinase K.Inorganic chemistry · 2021Article
- Lysozyme Improves the Inhibitory Effects ofFrontiers in nutrition · 2021Article
- Multivalent ions and biomolecules: Attempting a comprehensive perspective.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.Journal of applied crystallography · 2019Article
- The Chaotropic Effect as an Assembly Motif in Chemistry.Angewandte Chemie (International ed. in English) · 2018Review
- The P-type ATPase inhibiting potential of polyoxotungstates.Metallomics : integrated biometal science · 2018Article
- The antibacterial activity of polyoxometalates: structures, antibiotic effects and future perspectives.Chemical communications (Cambridge, England) · 2018Article
- Article
- Photoluminescence Properties of Two Closely Related Isostructural Series Based on Anderson-Evans Cluster Coordinated With Lanthanides [Ln(HFrontiers in chemistry · 2018Article
- Article
- Article
- Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography.Accounts of chemical research · 2017Review
- The "Sticky Patch" Model of Crystallization and Modification of Proteins for Enhanced Crystallizability.Methods in molecular biology (Clifton, N.J.) · 2017Review
- In situ formation of the first proteinogenically functionalized [TeWChemical communications (Cambridge, England) · 2016Article
- [Ni(OH)3W6O18(OCH2)3CCH2OH](4-): the first tris-functionalized Anderson-type heteropolytungstate.Chemical communications (Cambridge, England) · 2016Article
- Aurone synthase is a catechol oxidase with hydroxylase activity and provides insights into the mechanism of plant polyphenol oxidases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
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6 authors.
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Abstract
As synchrotron radiation becomes more intense, detectors become faster and structure-solving software becomes more elaborate, obtaining single crystals suitable for data collection is now the bottleneck in macromolecular crystallography. Hence, there is a need for novel and advanced crystallisation agents with the ability to crystallise proteins that are otherwise challenging. Here, an Anderson-Evans-type polyoxometalate (POM), specifically Na6 [TeW6 O24 ]⋅22 H2 O (TEW), is employed as a crystallisation additive. Its effects on protein crystallisation are demonstrated with hen egg-white lysozyme (HEWL), which co-crystallises with TEW in the vicinity (or within) the liquid-liquid phase separation (LLPS) region. The X-ray structure (PDB ID: 4PHI) determination revealed that TEW molecules are part of the crystal lattice, thus demonstrating specific binding to HEWL with electrostatic interactions and hydrogen bonds. The negatively charged TEW polyoxotungstate binds to sites with a positive electrostatic potential located between two (or more) symmetry-related protein chains. Thus, TEW facilitates the formation of protein-protein interfaces of otherwise repulsive surfaces, and thereby the realisation of a stable crystal lattice. In addition to retaining the isomorphicity of the protein structure, the anomalous scattering of the POMs was used for macromolecular phasing. The results suggest that hexatungstotellurate(VI) has great potential as a crystallisation additive to promote both protein crystallisation and structure elucidation.
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