ArticleJournal of experimental botany2015
Differential gene expression and alternative splicing between diploid and tetraploid watermelon.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- Polyploidization Redirects Carbon Flux to Diterpenoid Biosynthesis inPlants (Basel, Switzerland) · 2026Article
- Importance of pre-mRNA splicing and its study tools in plants.Advanced biotechnology · 2024Review
- Comprehensive Profiling of Alternative Splicing and Alternative Polyadenylation during Fruit Ripening in Watermelon (International journal of molecular sciences · 2023Article
- Current insights into posttranscriptional regulation of fleshy fruit ripening.Plant physiology · 2023Review
- Development of homozygous tetraploid potato and whole genome doubling-induced the enrichment of H3K27ac and potentially enhanced resistance to cold-induced sweetening in tubers.Horticulture research · 2023Article
- Whole genome doubling-induced the enrichment of H3K27me3 in genes carrying specific TEs inFrontiers in genetics · 2023Article
- A Comparative Study of Morphology, Photosynthetic Physiology, and Proteome between Diploid and Tetraploid Watermelon (Bioengineering (Basel, Switzerland) · 2022Article
- Genetic and Physiological Responses to Heat Stress inFrontiers in plant science · 2022Review
- The Transcriptional and Splicing Changes Caused by Hybridization Can Be Globally Recovered by Genome Doubling during Allopolyploidization.Molecular biology and evolution · 2021Article
- QTLs and candidate genes analyses for fruit size under domestication and differentiation in melon (Cucumis melo L.) based on high resolution maps.BMC plant biology · 2021Article
- Mapping and Analysis of a Novel Genic Male Sterility Gene in Watermelon (Frontiers in plant science · 2021Article
- Changes in Alternative Splicing in Response to Domestication and Polyploidization in Wheat.Plant physiology · 2020Article
- Transcriptome sequencing and screening of genes related to sex determination of Trichosanthes kirilowii Maxim.PloS one · 2020Article
- Genome-wide analysis of alternative splicing divergences between Brassica hexaploid and its parents.Planta · 2019Article
- Extensive changes in gene expression and alternative splicing due to homoeologous exchange in rice segmental allopolyploids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Transcriptome-based gene expression profiling of diploid radish (Raphanus sativus L.) and the corresponding autotetraploid.Molecular biology reports · 2019Article
- Transcriptome analysis of alternative splicing in peanut (Arachis hypogaea L.).BMC plant biology · 2018Article
- Article
- Which Genes in a Typical Intertidal Seagrass (Frontiers in plant science · 2018Article
- De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
Abstract
The exploitation of synthetic polyploids for producing seedless fruits is well known in watermelon. Tetraploid progenitors of triploid watermelon plants, compared with their diploid counterparts, exhibit wide phenotypic differences. Although many factors modulate alternative splicing (AS) in plants, the effects of autopolyploidization on AS are still unknown. In this study, we used tissues of leaf, stem, and fruit of diploid and tetraploid sweet watermelon to understand changes in gene expression and the occurrence of AS. RNA-sequencing analysis was performed along with reverse transcription quantitative PCR and rapid amplification of cDNA ends (RACE)-PCR to demonstrate changes in expression and splicing. All vegetative tissues except fruit showed an increased level of AS in the tetraploid watermelon throughout the growth period. The ploidy levels of diploids and the tetraploid were confirmed using a ploidy analyser. We identified 5362 and 1288 genes that were up- and downregulated, respectively, in tetraploid as compared with diploid plants. We further confirmed that 22 genes underwent AS events across tissues, indicating possibilities of generating different protein isoforms with altered functions of important transcription factors and transporters. Arginine biosynthesis, chlorophyllide synthesis, GDP mannose biosynthesis, trehalose biosynthesis, and starch and sucrose degradation pathways were upregulated in autotetraploids. Phloem protein 2, chloroplastic PGR5-like protein, zinc-finger protein, fructokinase-like 2, MYB transcription factor, and nodulin MtN21 showed AS in fruit tissues. These results should help in developing high-quality seedless watermelon and provide additional transcriptomic information related to other cucurbits.
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