ArticleNucleic acids research2015
Conformation transitions of eukaryotic polyribosomes during multi-round translation.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
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- Ribosomal RNA Degradation (RNA Disruption) in Tumour Cells: Mechanistic Insights and Potential Clinical Utility.Cancers · 2025Review
- Synthesis of Differentially Halogenated Lissoclimide Analogues To Probe Ribosome E-Site Binding.ACS chemical biology · 2025Article
- The impact of mRNA poly(A) tail length on eukaryotic translation stages.Nucleic acids research · 2024Article
- Polyribosomes of circular topology are prevalent in mammalian cells.Nucleic acids research · 2023Article
- Global Assessment of Protein Translation in Mammalian Cells Using Polysome Fractionation.Methods in molecular biology (Clifton, N.J.) · 2023Article
- mRNA- and factor-driven dynamic variability controls eIF4F-cap recognition for translation initiation.Nucleic acids research · 2022Article
- Smart-ORF: a single-molecule method for accessing ribosome dynamics in both upstream and main open reading frames.Nucleic acids research · 2021Article
- Functional Cyclization of Eukaryotic mRNAs.International journal of molecular sciences · 2020Article
- Revisiting the Closed-Loop Model and the Nature of mRNA 5'-3' Communication.Molecular cell · 2018Review
- mRNP architecture in translating and stress conditions reveals an ordered pathway of mRNP compaction.The Journal of cell biology · 2018Article
- Spatial Organization of Single mRNPs at Different Stages of the Gene Expression Pathway.Molecular cell · 2018Article
- ZNF598 Is a Quality Control Sensor of Collided Ribosomes.Molecular cell · 2018Article
- N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.Nucleic acids research · 2017Article
- Ribosome reinitiation can explain length-dependent translation of messenger RNA.PLoS computational biology · 2017Article
- Importins promote high-frequency NF-κB oscillations increasing information channel capacity.Biology direct · 2016Article
- Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.PLoS genetics · 2016Article
- Peering at Brain Polysomes with Atomic Force Microscopy.Journal of visualized experiments : JoVE · 2016Article
- Linking Α to Ω: diverse and dynamic RNA-based mechanisms to regulate gene expression by 5'-to-3' communication.F1000Research · 2016Review
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using sedimentation and cryo electron tomography techniques, the conformations of eukaryotic polyribosomes formed in a long-term cell-free translation system were analyzed over all the active system lifetime (20-30 translation rounds during 6-8 h in wheat germ extract at 25°C). Three distinct types of the conformations were observed: (i) circular polyribosomes, varying from ring-shaped forms to circles collapsed into double rows, (ii) linear polyribosomes, tending to acquire planar zigzag-like forms and (iii) densely packed 3D helices. At the start, during the first two rounds of translation mostly the circular (ring-shaped and double-row) polyribosomes and the linear (free-shaped and zigzag-like) polyribosomes were formed ('juvenile phase'). The progressive loading of the polyribosomes with translating ribosomes induced the opening of the circular polyribosomes and the transformation of a major part of the linear polyribosomes into the dense 3D helices ('transitional phase'). After 2 h from the beginning (about 8-10 rounds of translation) this compact form of polyribosomes became predominant, whereas the circular and linear polyribosome fractions together contained less than half of polysomal ribosomes ('steady-state phase'). The latter proportions did not change for several hours. Functional tests showed a reduced translational activity in the fraction of the 3D helical polyribosomes.
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