ArticleOrganic & biomolecular chemistry2015
Near-instant surface-selective fluorogenic protein quantification using sulfonated triarylmethane dyes and fluorogen activating proteins.
Article in Organic & biomolecular chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed.
- Chronic benzodiazepine treatment triggers gephyrin scaffold destabilization and GABAFrontiers in cellular neuroscience · 2025Article
- Optimization of the fluorogen-activating protein tag for quantitative protein trafficking and colocalization studies inMolecular biology of the cell · 2024Article
- Aberrant Topologies of Bacterial Membrane Proteins Revealed by High Sensitivity Fluorescence Labelling.Journal of molecular biology · 2024Article
- Temporally and spatially partitioned neuropeptide release from individual clock neurons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Protein Proximity Observed Using Fluorogen Activating Protein and Dye Activated by Proximal Anchoring (FAP-DAPA) System.ACS chemical biology · 2020Article
- Visualizing GABA A Receptor Trafficking Dynamics with Fluorogenic Protein Labeling.Current protocols in neuroscience · 2020Article
- Fluorogen activating protein toolset for protein trafficking measurements.Traffic (Copenhagen, Denmark) · 2020Review
- Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging.International journal of molecular sciences · 2019Review
- Activity-evoked and spontaneous opening of synaptic fusion pores.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Fluorogenic Photoaffinity Labeling of Proteins in Living Cells.Bioconjugate chemistry · 2019Article
- Article
- Select α-arrestins control cell-surface abundance of the mammalian Kir2.1 potassium channel in a yeast model.The Journal of biological chemistry · 2018Article
- Fluorogen-activating proteins: beyond classical fluorescent proteins.Acta pharmaceutica Sinica. B · 2018Review
- High-Content Surface and Total Expression siRNA Kinase Library Screen with VX-809 Treatment Reveals Kinase Targets that Enhance F508del-CFTR Rescue.Molecular pharmaceutics · 2018Article
- HIV-1 Envelope Glycoprotein Trafficking through the Endosomal Recycling Compartment Is Required for Particle Incorporation.Journal of virology · 2018Article
- A versatile optical tool for studying synaptic GABAJournal of cell science · 2017Article
- Fluorogenic Labeling Strategies for Biological Imaging.International journal of molecular sciences · 2017Review
- Super-Resolution Microscopy and Single-Protein Tracking in Live Bacteria Using a Genetically Encoded, Photostable Fluoromodule.Current protocols in cell biology · 2017Article
- Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals β4-Mediated Control of Channel Clustering in Cerebellum.Frontiers in cellular neuroscience · 2017Article
- Multiexcitation Fluorogenic Labeling of Surface, Intracellular, and Total Protein Pools in Living Cells.Bioconjugate chemistry · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Agonist-promoted G-protein coupled receptor (GPCR) endocytosis and recycling plays an important role in many signaling events in the cell. However, the approaches that allow fast and quantitative analysis of such processes still remain limited. Here we report an improved labeling approach based on the genetic fusion of a fluorogen activating protein (FAP) to a GPCR and binding of a sulfonated analog of the malachite green (MG) fluorogen to rapidly and selectively label cell surface receptors. Fluorescence microscopy and flow cytometry demonstrate that this dye does not cross the plasma membrane, binds with high affinity to a dL5** FAP-GPCR fusion construct, activating tagged surface receptors within seconds of addition. The ability to rapidly and selectively label cell surface receptors with a fluorogenic genetically encoded tag allows quantitative imaging and analysis of highly dynamic processes like receptor endocytosis and recycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.