ArticleThe EMBO journal2015
Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells.
Erratum issuedOpen access · bronzeAbstract read
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Article in The EMBO journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
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38citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
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Who cites it
38 citing papers in PubMed, 54 citations in OpenAlex.
- Extracellular Vesicles from Canine Mesenchymal Stem Cells-Isolation, Characterization and miRNA Definition Following Interleukin-1ß and Shockwave Treatment.Animals : an open access journal from MDPI · 2026Article
- Elucidation of how the Mir-23-27-24 cluster regulates development and aging.Experimental & molecular medicine · 2024Review
- Evidence for Functional Roles of MicroRNAs in Lineage Specification During Mouse and Human Preimplantation Development.The Yale journal of biology and medicine · 2023Review
- MicroRNAs Regulate CaCells · 2023Article
- Review
- A functional SNP rs895819 on pre-miR-27a is associated with bipolar disorder by targeting NCAM1.Communications biology · 2022Article
- Non-Coding RNAs as Regulators of Myogenesis and Postexercise Muscle Regeneration.International journal of molecular sciences · 2021Review
- Article
- The miR-23a∼27a∼24-2 microRNA Cluster Promotes Inflammatory Polarization of Macrophages.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Post-transcriptional regulation of MRTF-A by miRNAs during myogenic differentiation of myoblasts.Nucleic acids research · 2020Article
- The Key Role of MicroRNAs in Self-Renewal and Differentiation of Embryonic Stem Cells.International journal of molecular sciences · 2020Review
- Functional omics analyses reveal only minor effects of microRNAs on human somatic stem cell differentiation.Scientific reports · 2020Article
- Functional Dissection of pri-miR-290~295 in Dgcr8 Knockout Mouse Embryonic Stem Cells.International journal of molecular sciences · 2019Article
- High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation.Stem cell research & therapy · 2019Article
- Dgcr8 knockout approaches to understand microRNA functions in vitro and in vivo.Cellular and molecular life sciences : CMLS · 2019Review
- An Insight into DNA-free Reprogramming Approaches to Generate Integration-free Induced Pluripotent Stem Cells for Prospective Biomedical Applications.Stem cell reviews and reports · 2019Review
- Arrayed functional genetic screenings in pluripotency reprogramming and differentiation.Stem cell research & therapy · 2019Review
- MiR-222-3p in Platelets Serves as a Distinguishing Marker for Early Recognition of Kawasaki Disease.Frontiers in pediatrics · 2019Article
- Protein arginine methyltransferase 7-mediatedThe Journal of biological chemistry · 2018Article
- The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation.Experimental hematology · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 2 institutions in 1 country.
Yanni MaState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Nan YaoState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Guang LiuState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Lei DongState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Yufang LiuState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Meili ZhangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Fang WangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Bin WangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Xueju WeiState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
He DongState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Lanlan WangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Shaowei JiState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Junwu ZhangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
Yangming WangPeking-Tsinghua Joint Center for Life Sciences, Institute of Molecular Medicine, Peking University, Beijing, China.
Yue HuangState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Medical Genetics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China huangyue@pumc.edu.cn j-yu@ibms.pumc.edu.cn.
Jia YuState Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China huangyue@pumc.edu.cn j-yu@ibms.pumc.edu.cn.
Chinese Academy of Medical Sciences & Peking Union Medical College · CNCenter for Life Sciences · CN
Funding
Wellcome Trust
Abstract
MicroRNAs play important roles in controlling the embryonic stem cell (ESC) state. Although much is known about microRNAs maintaining ESC state, microRNAs that are responsible for promoting ESC differentiation are less reported. Here, by screening 40 microRNAs pre-selected by their expression patterns and predicted targets in Dgcr8-null ESCs, we identify 14 novel differentiation-associated microRNAs. Among them, miR-27a and miR-24, restrained by c-Myc in ESC, exert their roles of silencing self-renewal through directly targeting several important pluripotency-associated factors, such as Oct4, Foxo1 and Smads. CRISPR/Cas9-mediated knockout of all miR-27/24 in ESCs leads to serious deficiency in ESC differentiation in vitro and in vivo. Moreover, depleting of them in mouse embryonic fibroblasts can evidently promote somatic cell reprogramming. Altogether, our findings uncover the essential role of miR-27 and miR-24 in ESC differentiation and also demonstrate novel microRNAs responsible for ESC differentiation.
Indexed as
AnimalsCell DifferentiationEmbryonic Stem CellsForkhead Box Protein O1Forkhead Transcription FactorsHEK293 CellsHumansMiceMice, KnockoutMicroRNAsNIH 3T3 CellsOctamer Transcription Factor-3Proto-Oncogene Proteins c-mycRNA-Binding ProteinsSmad ProteinsDgcr8 protein, mouseForkhead Box Protein O1Forkhead Transcription FactorsFoxo1 protein, mouseMicroRNAsMirn24 microRNA, mouseMirn27 microRNA, mouseMyc protein, mouseOctamer Transcription Factor-3Pou5f1 protein, mouseProto-Oncogene Proteins c-mycRNA-Binding ProteinsSmad Proteinsc‐Mycdifferentiationembryonic stem cellsiPSC generationmicroRNA
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.