ArticleThe Journal of biological chemistry2015
The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Engineered extracellular vesicles for combinatorial cancer therapy and imaging.Acta pharmaceutica Sinica. B · 2026Review
- Peroxisome-derived ether lipids regulate lysosomal exocytosis.The EMBO journal · 2026Article
- Multiomics analysis to evaluate the enrichment of extracellular vesicles from human plasma.Journal of lipid research · 2025Article
- Review
- Native and Engineered Extracellular Vesicles for the Treatment of Acute Lung Injury and Acute Respiratory Distress Syndrome.Small science · 2025Article
- Quantitative Lipid Analysis of Extracellular Vesicle Preparations: A Perspective.Journal of extracellular vesicles · 2025Article
- Isolation, identification, and challenges of extracellular vesicles: emerging players in clinical applications.Apoptosis : an international journal on programmed cell death · 2025Review
- Article
- Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers.Biochemistry and biophysics reports · 2024Article
- Intercellular transfer of cancer cell invasiveness via endosome-mediated protease shedding.Nature communications · 2024Article
- Extracellular vesicles for developing targeted hearing loss therapy.Journal of controlled release : official journal of the Controlled Release Society · 2024Review
- Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk.Journal of extracellular vesicles · 2024Article
- Progression in the Relationship between Exosome Production and Atherosclerosis.Current pharmaceutical biotechnology · 2024Review
- Exosomes Interactions with the Blood-Brain Barrier: Implications for Cerebral Disorders and Therapeutics.International journal of molecular sciences · 2023Review
- Lipids in Extracellular Vesicles: What Can Be Learned about Membrane Structure and Function?Cold Spring Harbor perspectives in biology · 2023Review
- Liquid Biopsy at the Frontier of Kidney Diseases: Application of Exosomes in Diagnostics and Therapeutics.Genes · 2023Review
- siRNA screening reveals that SNAP29 contributes to exosome release.Cellular and molecular life sciences : CMLS · 2023Article
- Lipidomic landscape of circulating extracellular vesicles isolated from adolescents exposed to ethanol intoxication: a sex difference study.Biology of sex differences · 2023Article
- The lipid composition of extracellular vesicles: applications in diagnostics and therapeutic delivery.Frontiers in molecular biosciences · 2023Review
- Extracellular vesicles in β cell biology: Role of lipids in vesicle biogenesis, cargo, and intercellular signaling.Molecular metabolism · 2022Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exosomes are vesicles released by cells after fusion of multivesicular bodies with the plasma membrane. In this study, we have investigated whether ether lipids affect the release of exosomes in PC-3 cells. To increase the cellular levels of ether lipids, the ether lipid precursor hexadecylglycerol was added to cells. Lipidomic analysis showed that this compound was in fact able to double the cellular levels of ether lipids in these cells. Furthermore, increased levels of ether lipids were also found in exosomes released by cells containing high levels of these lipids. Interestingly, as measured by nanoparticle tracking analysis, cells containing high levels of ether lipids released more exosomes than control cells, and these exosomes were similar in size to control exosomes. Moreover, silver staining and Western blot analyses showed that the protein composition of exosomes released in the presence of hexadecylglycerol was changed; the levels of some proteins were increased, and the levels of others were reduced. In conclusion, this study clearly shows that an increase in cellular ether lipids is associated with changes in the release and composition of exosomes.
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