ArticleLab on a chip2015
Parallel RNA extraction using magnetic beads and a droplet array.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Programmable magnetic robot (ProMagBot) for automated nucleic acid extraction at the point of need.Lab on a chip · 2023Article
- Magnetic Bead Manipulation in Microfluidic Chips for Biological Application.Cyborg and bionic systems (Washington, D.C.) · 2023Article
- Rapid nucleic acid extraction from skin biopsies using a point-of-care device.Lab on a chip · 2022Article
- Droplet Manipulation under a Magnetic Field: A Review.Biosensors · 2022Review
- Research on magnetic bead motion characteristics based on magnetic beads preset technology.Scientific reports · 2021Article
- Facile Coupling of Droplet Magnetofluidic-Enabled Automated Sample Preparation for Digital Nucleic Acid Amplification Testing and Analysis.Analytical chemistry · 2020Article
- Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2020Review
- Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting.Trends in analytical chemistry : TRAC · 2020Review
- Continuous Microfluidic Purification of DNA Using Magnetophoresis.Micromachines · 2020Article
- Integrated microfluidic systems with sample preparation and nucleic acid amplification.Lab on a chip · 2019Review
- Microfluidic communicating vessel chip for expedited and automated immunomagnetic assays.Lab on a chip · 2018Article
- Moving toward rapid and low-cost point-of-care molecular diagnostics with a repurposed 3D printer and RPA.Analytical biochemistry · 2018Article
- Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis.Scientific reports · 2016Article
- Low-Cost 3D Printers Enable High-Quality and Automated Sample Preparation and Molecular Detection.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid extraction is a necessary step for most genomic/transcriptomic analyses, but it often requires complicated mechanisms to be integrated into a lab-on-a-chip device. Here, we present a simple, effective configuration for rapidly obtaining purified RNA from low concentration cell medium. This Total RNA Extraction Droplet Array (TREDA) utilizes an array of surface-adhering droplets to facilitate the transportation of magnetic purification beads seamlessly through individual buffer solutions without solid structures. The fabrication of TREDA chips is rapid and does not require a microfabrication facility or expertise. The process takes less than 5 minutes. When purifying mRNA from bulk marine diatom samples, its repeatability and extraction efficiency are comparable to conventional tube-based operations. We demonstrate that TREDA can extract the total mRNA of about 10 marine diatom cells, indicating that the sensitivity of TREDA approaches single-digit cell numbers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.