ArticleNature communications2014
Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 168 citations in OpenAlex.
- SPIFFI enables single-shot super-resolution and multidimensional imaging.Nature methods · 2026Article
- Three-Dimensional Optical and Computational Reconstruction of Colloidal Electrokinetic Flows via Multiplane Imaging and Multiscale Modeling.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Multiplane 2.5D microscopy for high-throughput high-resolution tissue imaging.Journal of biomedical optics · 2025Article
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- SOGO-SOFI, light-modulated super-resolution optical fluctuation imaging using only 20 raw frames for high-fidelity reconstruction.Fundamental research · 2025Article
- High-Throughput Single-Molecule Microscopy with Adaptable Spatial Resolution Using Exchangeable Oligonucleotide Labels.ACS nano · 2025Article
- Multifocal microscopy for functional imaging of neural systems.Neurophotonics · 2024Article
- Fast volumetric multifocus structured illumination microscopy of subcellular dynamics in living cells.Biomedical optics express · 2024Article
- High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation.Nature methods · 2023Article
- Multiplane HiLo microscopy with speckle illumination and non-local means denoising.Journal of biomedical optics · 2023Article
- Article
- Learning flat optics for extended depth of field microscopy imaging.Nanophotonics (Berlin, Germany) · 2023Article
- Live-Cell SOFI Correlation with SMLM and AFM Imaging.ACS bio & med chem Au · 2023Article
- Spatially Resolving Size Effects on Diffusivity in Nanoporous Extracellular Matrix-like Materials with Fluorescence Correlation Spectroscopy Super-Resolution Optical Fluctuation Imaging.The journal of physical chemistry. B · 2023Article
- Unravelling 3D Dynamics and Hydrodynamics during Incorporation of Dielectric Particles to an Optical Trapping Site.ACS nano · 2023Article
- Optical-resolution photoacoustic microscopy with a needle-shaped beam.Nature photonics · 2023Article
- Fluorescence fluctuation-based super-resolution microscopy: Basic concepts for an easy start.Journal of microscopy · 2022Review
- Article
- Article
- Approach to map nanotopography of cell surface receptors.Communications biology · 2022Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefield microscope. Previously, three-dimensional (3D) SOFI has been demonstrated by sequential imaging of multiple depth positions. Here we introduce a multiplexed imaging scheme for the simultaneous acquisition of multiple focal planes. Using 3D cross-cumulants, we show that the depth sampling can be increased. The simultaneous acquisition of multiple focal planes significantly reduces the acquisition time and thus the photobleaching. We demonstrate multiplane 3D SOFI by imaging fluorescently labelled cells over an imaged volume of up to 65 × 65 × 3.5 μm(3) without depth scanning. In particular, we image the 3D network of mitochondria in fixed C2C12 cells immunostained with Alexa 647 fluorophores and the 3D vimentin structure in living Hela cells expressing the fluorescent protein Dreiklang.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.