ArticleTissue engineering. Part C, Methods2015
Noninvasive Quantitative Imaging of Collagen Microstructure in Three-Dimensional Hydrogels Using High-Frequency Ultrasound.
Article in Tissue engineering. Part C, Methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Quantitative Ultrasonographic Assessment of Supraspinatus Insertion Tendon in Non-Lame Dogs (Thickness and Relative Echogenicity).Animals : an open access journal from MDPI · 2026Article
- Dual-Frequency Ultrasound Enhances Cavitation of Microdroplets for Controlled Scaffold Porosity in Tissue Engineering.ACS applied materials & interfaces · 2026Article
- Characterizing tendon microstructure using metrics associated with the angular dependence of ultrasound backscatter.The Journal of the Acoustical Society of America · 2026Article
- Microwave-assisted immunostaining for rapid labeling of matrix-embedded multicellular structures.APL bioengineering · 2025Article
- Echo intensity and gray-level co-occurrence matrix analysis of soft tissue grafting biomaterials and dental implants: anDento maxillo facial radiology · 2023Article
- In vivo acoustic patterning of endothelial cells for tissue vascularization.Scientific reports · 2023Article
- Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair.Advanced healthcare materials · 2022Article
- Collagen Remodeling along Cancer Progression Providing a Novel Opportunity for Cancer Diagnosis and Treatment.International journal of molecular sciences · 2022Review
- Effect of Thrombin and Incubation Time on Porcine Whole Blood Clot Elasticity and Recombinant Tissue Plasminogen Activator Susceptibility.Ultrasound in medicine & biology · 2022Article
- Genipin-Based Crosslinking of Jellyfish Collagen 3D Hydrogels.Gels (Basel, Switzerland) · 2021Article
- Medical imaging of tissue engineering and regenerative medicine constructs.Biomaterials science · 2021Review
- A novel quantitative and reference-free ultrasound analysis to discriminate different concentrations of bone mineral content.Scientific reports · 2021Article
- Imaging methods to evaluate tumor microenvironment factors affecting nanoparticle drug delivery and antitumor response.Cancer drug resistance (Alhambra, Calif.) · 2021Article
- Using Acoustic Fields to Fabricate ECM-Based Biomaterials for Regenerative Medicine Applications.Recent progress in materials · 2020Article
- A history of exploring cancer in context.Nature reviews. Cancer · 2018Review
- Characterization of the Tumor Microenvironment and Tumor-Stroma Interaction by Non-invasive Preclinical Imaging.Frontiers in oncology · 2017Review
- Ultrasound patterning technologies for studying vascular morphogenesis in 3D.Journal of cell science · 2017Article
- Ultrasound Imaging Techniques for Spatiotemporal Characterization of Composition, Microstructure, and Mechanical Properties in Tissue Engineering.Tissue engineering. Part B, Reviews · 2016Review
- Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials.Annals of biomedical engineering · 2016Review
- Development of a High-Throughput Ultrasound Technique for the Analysis of Tissue Engineering Constructs.Annals of biomedical engineering · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Collagen I is widely used as a natural component of biomaterials for both tissue engineering and regenerative medicine applications. The physical and biological properties of fibrillar collagens are strongly tied to variations in collagen fiber microstructure. The goal of this study was to develop the use of high-frequency quantitative ultrasound to assess collagen microstructure within three-dimensional (3D) hydrogels noninvasively and nondestructively. The integrated backscatter coefficient (IBC) was employed as a quantitative ultrasound parameter to detect, image, and quantify spatial variations in collagen fiber density and diameter. Collagen fiber microstructure was varied by fabricating hydrogels with different collagen concentrations or polymerization temperatures. IBC values were computed from measurements of the backscattered radio-frequency ultrasound signals collected using a single-element transducer (38-MHz center frequency, 13-47 MHz bandwidth). The IBC increased linearly with increasing collagen concentration and decreasing polymerization temperature. Parametric 3D images of the IBC were generated to visualize and quantify regional variations in collagen microstructure throughout the volume of hydrogels fabricated in standard tissue culture plates. IBC parametric images of corresponding cell-embedded collagen gels showed cell accumulation within regions having elevated collagen IBC values. The capability of this ultrasound technique to noninvasively detect and quantify spatial differences in collagen microstructure offers a valuable tool to monitor the structural properties of collagen scaffolds during fabrication, to detect functional differences in collagen microstructure, and to guide fundamental research on the interactions of cells and collagen matrices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.