ArticleAmerican journal of respiratory cell and molecular biology2015
Smoking-Associated Site-Specific Differential Methylation in Buccal Mucosa in the COPDGene Study.
Article in American journal of respiratory cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- Evaluation of commonly used analysis strategies for epigenome- and transcriptome-wide association studies through replication of large-scale population studies.Genome biology · 2019Pooled it
- A whole-blood transcriptome meta-analysis identifies gene expression signatures of cigarette smoking.Human molecular genetics · 2016Pooled it
- Epigenetic Signatures of Cigarette Smoking.Circulation. Cardiovascular genetics · 2016Pooled it
- DNA Methylation in Newborns and Maternal Smoking in Pregnancy: Genome-wide Consortium Meta-analysis.American journal of human genetics · 2016Pooled it
- Improvements in lung function following vitamin C supplementation to pregnant smokers are associated with buccal DNA methylation at 5 years of age.Clinical epigenetics · 2024Trial
- Reduced mouse allergen is associated with epigenetic changes in regulatory genes, but not mouse sensitization, in asthmatic children.Environmental research · 2017Trial
- Attenuated FTO induces necroptosis of alveolar epithelium via the mRedox biology · 2026Article
- The cell with many faces: lung macrophage plasticity and function in response to environmental and pathogenic insults.Physiological reviews · 2026Review
- Epigenetic dysregulation of IRF9 drives excessive interferon signaling in COPD.EMBO molecular medicine · 2026Article
- Cigarette Smoking and E-cigarette Use Induce Shared DNA Methylation Changes Linked to Carcinogenesis.Cancer research · 2024Article
- High-resolution transcriptomic and epigenetic profiling identifies novel regulators of COPD.The EMBO journal · 2023Article
- How and why tobacco use affects reconstructive surgical practice: a contemporary narrative review.Translational andrology and urology · 2023Review
- Differential Methylation of Chronic Obstructive Pulmonary Disease Lung Macrophage Genes Sheds Light on Disease Pathogenesis.American journal of respiratory cell and molecular biology · 2022Article
- Sex and Gender Omic Biomarkers in Men and Women With COPD: Considerations for Precision Medicine.Chest · 2021Review
- Genetic risk factors for colorectal cancer in multiethnic Indonesians.Scientific reports · 2021Article
- Epigenome-wide association study on asthma and chronic obstructive pulmonary disease overlap reveals aberrant DNA methylations related to clinical phenotypes.Scientific reports · 2021Article
- Changes in global DNA methylation and hydroxymethylation in oral mucosa according to tobacco smoke exposure.The Journal of international medical research · 2020Article
- DNA Methylation Is Predictive of Mortality in Current and Former Smokers.American journal of respiratory and critical care medicine · 2020Article
- Epigenetic Aging: More Than Just a Clock When It Comes to Cancer.Cancer research · 2020Review
- Omics and the Search for Blood Biomarkers in Chronic Obstructive Pulmonary Disease. Insights from COPDGene.American journal of respiratory cell and molecular biology · 2019Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
DNA methylation is a complex, tissue-specific phenomenon that can reflect both endogenous factors and exogenous exposures. Buccal brushings represent an easily accessible source of DNA, which may be an appropriate surrogate tissue in the study of environmental exposures and chronic respiratory diseases. Buccal brushings were obtained from a subset of current and former smokers from the COPDGene study. Genome-wide DNA methylation data were obtained in the discovery cohort (n = 82) using the Illumina HumanMethylation450K array. Empirical Bayes methods were used to test for differential methylation by current smoking status at 468,219 autosomal CpG sites using linear models adjusted for age, sex, and race. Pyrosequencing was performed in a nonoverlapping replication cohort (n = 130). Current smokers were significantly younger than former smokers in both the discovery and replication cohorts. Seven CpG sites were associated with current smoking at a false discovery rate less than 0.05 in the discovery cohort. Six of the seven significant sites were pyrosequenced in the replication cohort; five CpG sites, including sites annotated to CYP1B1 and PARVA, were replicated. Correlations between cumulative smoke exposure and time since smoking cessation were observed in a subset of the significantly associated CpG sites. A significant correlation between reduced lung function and increased radiographic emphysema with methylation at cg02162897 (CYP1B1) was observed among female subjects. Site-specific methylation of DNA isolated from buccal mucosa is associated with exposure to cigarette smoke, and may provide insights into the mechanisms underlying differential susceptibility toward the development of smoking-related chronic respiratory diseases.
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