ArticleArquivos brasileiros de cardiologia2015
Role of microRNAs 221/222 on statin induced nitric oxide release in human endothelial cells.
Article in Arquivos brasileiros de cardiologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Pleiotropic Effects of Statins: Focus on Endothelial Function, Plaque Stability and Thrombosis (Part II).Current atherosclerosis reports · 2026Review
- Predictors of Coronary Collateral Circulation in Patients with Acute ST-segment Elevation Myocardial Infarction: A Nomogram-based Approach.Reviews in cardiovascular medicine · 2025Article
- Non-Anticoagulation Strategies Aimed at Primary Stroke Prevention in Nascent Atrial Fibrillation.Biomedicines · 2025Review
- Impact of statin therapy on CD40:CD40L signaling: mechanistic insights and therapeutic opportunities.Pharmacological reports : PR · 2025Review
- The pleiotropic effects of statins: a comprehensive exploration of neurovascular unit modulation and blood-brain barrier protection.Molecular medicine (Cambridge, Mass.) · 2024Review
- Plasma microRNAs as Biomarkers for Predicting Radiotherapy Treatment-Induced Cardiotoxicity in Lung Cancer.Life (Basel, Switzerland) · 2024Article
- The Role of Endothelial Progenitor Cells in Atherosclerosis and Impact of Anti-Lipemic Treatments on Endothelial Repair.International journal of molecular sciences · 2022Review
- COVID-19: Direct and Indirect Mechanisms of Statins.International journal of molecular sciences · 2021Review
- Statins in patients with COVID-19: a retrospective cohort study in Iranian COVID-19 patients.Translational medicine communications · 2021Article
- MicroRNAs hsa-miR-618 and hsa-miR-297 Might Modulate the Pleiotropic Effects Exerted by Statins in Endothelial Cells Through the Inhibition of ROCK2 Kinase:Frontiers in cardiovascular medicine · 2021Article
- Emerging Challenges of Radiation-Associated Cardiovascular Dysfunction (RACVD) in Modern Radiation Oncology: Clinical Practice, Bench Investigation, and Multidisciplinary Care.Frontiers in cardiovascular medicine · 2020Review
- Statin-Induced Nitric Oxide Signaling: Mechanisms and Therapeutic Implications.Journal of clinical medicine · 2019Review
- Pitavastatin nanoparticle-engineered endothelial progenitor cells repair injured vessels.Scientific reports · 2017Article
- The Lack of Clinical Applications Would be the Cause of Low Interest in an Endothelial Dysfunction Classification.Arquivos brasileiros de cardiologia · 2017Article
- Endothelial progenitor cells: Exploring the pleiotropic effects of statins.World journal of cardiology · 2017Review
- Pharmacogenetic Foundations of Therapeutic Efficacy and Adverse Events of Statins.International journal of molecular sciences · 2017Review
- Role of MicroRNA in Endothelial Dysfunction and Hypertension.Current hypertension reports · 2016Review
- MicroRNAs in Hyperglycemia Induced Endothelial Cell Dysfunction.International journal of molecular sciences · 2016Article
- MiR-135a Suppresses Calcification in Senescent VSMCs by Regulating KLF4/STAT3 Pathway.Current vascular pharmacology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNitric oxide (NO) has been largely associated with cardiovascular protection through improvement of endothelial function. Recently, new evidence about modulation of NO release by microRNAs (miRs) has been reported, which could be involved with statin-dependent pleiotropic effects, including anti-inflammatory properties related to vascular endothelium function.
objectiveTo evaluate the effects of cholesterol-lowering drugs including the inhibitors of cholesterol synthesis, atorvastatin and simvastatin, and the inhibitor of cholesterol absorption ezetimibe on NO release, NOS3 mRNA expression and miRs potentially involved in NO bioavailability.
methodsHuman umbilical vein endothelial cells (HUVEC) were exposed to atorvastatin, simvastatin or ezetimibe (0 to 5.0 μM). Cells were submitted to total RNA extraction and relative quantification of NOS3 mRNA and miRs -221, -222 and -1303 by qPCR. NO release was measured in supernatants by ozone-chemiluminescence.
resultsBoth statins increased NO levels and NOS3 mRNA expression but no influence was observed for ezetimibe treatment. Atorvastatin, simvastatin and ezetimibe down-regulated the expression of miR-221, whereas miR-222 was reduced only after the atorvastatin treatment. The magnitude of the reduction of miR-221 and miR-222 after treatment with statins correlated with the increment in NOS3 mRNA levels. No influence was observed on the miR-1303 expression after treatments.
conclusionNO release in endothelial cells is increased by statins but not by the inhibitor of cholesterol absorption, ezetimibe. Our results provide new evidence about the participation of regulatory miRs 221/222 on NO release induction mediated by statins. Although ezetimibe did not modulate NO levels, the down-regulation of miR-221 could involve potential effects on endothelial function.
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