ArticleMolecules (Basel, Switzerland)2014
Evaluation of the efficiency of different disruption methods on yeast cell wall preparation for β-glucan isolation.
Article in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Spent brewer's yeast as a source of high added value molecules: a systematic review on its characteristics, processing and potential applications.World journal of microbiology & biotechnology · 2020Pooled it
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- Engineering Strategies for Fungal Cell Disruption in Biotechnological Applications.Engineering in life sciences · 2025Review
- Pichia kudriavzevii yeast cell wall as a novel source of β-glucan: Extraction, characterization, and prebiotic functionality.Folia microbiologica · 2025Article
- Beta-Glucans in Biotechnology: A Holistic Review with a Special Focus on Yeast.Bioengineering (Basel, Switzerland) · 2025Review
- Release of Biopolymers fromMicroorganisms · 2024Article
- Spent Brewer's Yeast Lysis Enables aInternational journal of molecular sciences · 2024Review
- Functional characterization of biodegradable films obtained from whole Paecilomyces variotii biomass.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- A New Method for Selective Extraction of Torularhodin from Red Yeast Using COApplied biochemistry and biotechnology · 2024Article
- Hydrothermal conditioning of oleaginous yeast cells to enable recovery of lipids as potential drop-in fuel precursors.Biotechnology for biofuels and bioproducts · 2024Article
- A Comparative Study of Composition and Soluble Polysaccharide Content between Brewer's Spent Yeast and Cultured Yeast Cells.Foods (Basel, Switzerland) · 2024Article
- Adsorption of Zearalenone byToxins · 2024Article
- Assessment of mycotoxin sequestration efficacy in Saccharomyces cerevisiae by-products cultured in wheat bran and whey protein medium.Scientific reports · 2024Article
- Non-Current research in food science · 2024Article
- Solid-StateMolecules (Basel, Switzerland) · 2023Article
- Review
- FT-IR Method Limitations for β-Glucan Analysis.Molecules (Basel, Switzerland) · 2022Article
- Acceleration of Yeast Autolysis by Addition of Fatty Acids, Ethanol and Alkaline Protease.Iranian journal of biotechnology · 2022Article
- Functionality of Yeast β-Glucan Recovered fromPolymers · 2022Article
- Modulating Oral Delivery and Gastrointestinal Kinetics of Recombinant Proteins via Engineered Fungi.The AAPS journal · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selected methods for yeast cell disruption were evaluated to establish their suitability for cell wall preparation in the process of β-glucan isolation. The effect of different disruption methods on contents of total saccharides, β-glucans and proteins in the produced cell walls preparations was analyzed. The degree of cell wall purification from intracellular components was established on the basis of the ratio of solubilised material. The investigated methods included: cell exposure to hot water (autoclaving), thermally-induced autolysis, homogenization in a bead mill, sonication and their combinations. Experimental systems were prepared in water (pH 5.0 and pH 7.0) and Tris-HCl buffer (pH 8.0). The Saccharomyces cerevisiae yeast cell wall preparations with the highest degree of cytosol component release and purification of β-glucans were produced by 30 min of cell homogenization with zirconium-glass beads (0.5 mm in diameter). This was confirmed by the highest ratio of solubilised material (approx. 64%-67%). The thus-produced preparations contained ca. 60% of total saccharides, 13%-14% of β(1,3)/(1,6)-glucans, and approx. 35% of crude proteins. Similar results were obtained after autolysis coupled with bead milling as well as with sonication, but the time required for these processes was more than 24 h. Homogenization in a bead mill could be valuable for general isolation procedures because allows one to eliminate the different autolytic activity of various yeast strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.