ArticleBiophysical journal2014
Tetracycline determines the conformation of its aptamer at physiological magnesium concentrations.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mechanism underlying the high regulatory performance of the doxycycline riboswitch G12.Nature communications · 2026Article
- Aptamer-linked immobilized sorbent assay for detection of VP1 of foot and mouth disease virus serotype O.Scientific reports · 2026Article
- Beyond the niche - unlocking the full potential of synthetic riboswitches.Nature communications · 2025Review
- Antibody and Aptamer-Based Magnetic-Graphene Oxide Desorption (M-GOD) Quantum Dot Assays for Rapid and Sensitive Detection of SAR-CoV-2.Journal of fluorescence · 2025Article
- Interaction of Selected Anthracycline and Tetracycline Chemotherapeutics with Poly(I:C) Molecules.ACS omega · 2025Article
- Elucidating the molecular docking and binding dynamics of aptamers with spike proteins across SARS-CoV-2 variants of concern.Frontiers in microbiology · 2025Article
- Elimination of editing plasmid mediated by theophylline riboswitch in Zymomonas mobilis.Applied microbiology and biotechnology · 2023Article
- Tapping the potential of synthetic riboswitches: reviewing the versatility of the tetracycline aptamer.RNA biology · 2023Review
- Fluorescent Aptasensor and Colorimetric Aptablot for p-tau231 Detection: Toward Early Diagnosis of Alzheimer's Disease.Biomedicines · 2022Article
- What defines a synthetic riboswitch? - Conformational dynamics of ciprofloxacin aptamers with similar binding affinities but varying regulatory potentials.Nucleic acids research · 2021Article
- High-Throughput Amenable MALDI-MS Detection of RNA and DNA with On-Surface Analyte Enrichment Using Fluorous Partitioning.SLAS discovery : advancing life sciences R & D · 2021Article
- Inhibition of enteropathogenic Escherichia coli biofilm formation by DNA aptamer.Molecular biology reports · 2020Article
- Influence of MgRNA (New York, N.Y.) · 2019Article
- Applicability of a computational design approach for synthetic riboswitches.Nucleic acids research · 2017Article
- StreAM-[Formula: see text]: algorithms for analyzing coarse grained RNA dynamics based on Markov models of connectivity-graphs.Algorithms for molecular biology : AMB · 2017Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Synthetic riboswitches are versatile tools for the study and manipulation of biological systems. Yet, the underlying mechanisms governing its structural properties and regulation under physiological conditions are poorly studied. We performed spectroscopic and calorimetric experiments to explore the folding kinetics and thermodynamics of the tetracycline-binding aptamer, which can be employed as synthetic riboswitch, in the range of physiological magnesium concentrations. The dissociation constant of the ligand-aptamer complex was found to strongly depend on the magnesium concentration. At physiological magnesium concentrations, tetracycline induces a significant conformational shift from a compact, but heterogeneous intermediate state toward the completely formed set of tertiary interactions defining the regulation-competent structure. Thus, the switching functionality of the tetracycline-binding aptamer appears to include both a conformational rearrangement toward the regulation-competent structure and its thermodynamic stabilization.
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