ReviewBiophysical journal2014
Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state.
Review in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 82 citations in OpenAlex.
- Lipid network crosslinked hydrogels control material dynamics across multiple length scales through lipid movement.Nature communications · 2026Article
- Laurdan: Clarifying Photophysics and Advancing the Characterization of Extracellular Vesicles.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- "Head-to-Toe" Lipid Properties Govern the Binding and Cargo Transfer of High-Density Lipoprotein.Membranes · 2024Article
- Can calmodulin bind to lipids of the cytosolic leaflet of plasma membranes?Open biology · 2024Article
- Time-resolved fluorescence of tryptophan characterizes membrane perturbation by cyclic lipopeptides.Biophysical journal · 2024Article
- Laurdan Discerns Lipid Membrane Hydration and Cholesterol Content.The journal of physical chemistry. B · 2023Article
- Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions.Biomolecules · 2022Article
- Encapsulation of Vitamin C by Glycerol-Derived Dendrimers, Their Interaction with Biomimetic Models ofMolecules (Basel, Switzerland) · 2022Article
- Dissecting the mechanisms of environment sensitivity of smart probes for quantitative assessment of membrane properties.Open biology · 2022Article
- Lipid Melting Transitions Involve Structural Redistribution of Interfacial Water.The journal of physical chemistry. B · 2021Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- What Does Time-Dependent Fluorescence Shift (TDFS) in Biomembranes (and Proteins) Report on?Frontiers in chemistry · 2021Review
- Imaging non-classical mechanical responses of lipid membranes using molecular rotors.Chemical science · 2020Article
- Identification of the Molecular Determinants Involved in Antimicrobial Activity of Pseudodesmin A, a Cyclic Lipopeptide From the Viscosin Group.Frontiers in microbiology · 2020Article
- Long-Range Energy Transfer in Protein Megamolecules.Journal of the American Chemical Society · 2018Article
- Direct Prediction of EPR Spectra from Lipid Bilayers: Understanding Structure and Dynamics in Biological Membranes.Chemphyschem : a European journal of chemical physics and physical chemistry · 2018Article
- Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy.Biophysical journal · 2017Article
- Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane.Journal of physics D: Applied physics · 2017Article
- Using spectral decomposition of the signals from laurdan-derived probes to evaluate the physical state of membranes in live cells.F1000Research · 2017Article
- Solvatochromic fluorene-linked nucleoside and DNA as color-changing fluorescent probes for sensing interactions.Chemical science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence methods are versatile tools for obtaining dynamic and topological information about biomembranes because the molecular interactions taking place in lipid membranes frequently occur on the same timescale as fluorescence emission. The fluorescence intensity decay, in particular, is a powerful reporter of the molecular environment of a fluorophore. The fluorescence lifetime can be sensitive to the local polarity, hydration, viscosity, and/or presence of fluorescence quenchers/energy acceptors within several nanometers of the vicinity of a fluorophore. Illustrative examples of how time-resolved fluorescence measurements can provide more valuable and detailed information about a system than the time-integrated (steady-state) approach will be presented in this review: 1), determination of membrane polarity and mobility using time-dependent spectral shifts; 2), identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidation of membrane leakage mechanisms from dye self-quenching assays; and 4), evaluation of nanodomain sizes by time-resolved Förster resonance energy transfer measurements.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.