ArticleThe EMBO journal1989
Overproduction of the regulatory subunit of the cAMP-dependent protein kinase blocks the differentiation of Dictyostelium discoideum.
Article in The EMBO journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 74 citations in OpenAlex.
- A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.PloS one · 2013Article
- Evolution of developmental cyclic adenosine monophosphate signaling in the Dictyostelia from an amoebozoan stress response.Development, growth & differentiation · 2011Review
- Constitutively active protein kinase A disrupts motility and chemotaxis in Dictyostelium discoideum.Eukaryotic cell · 2003Article
- Role of PKA in the timing of developmental events in Dictyostelium cells.Microbiology and molecular biology reviews : MMBR · 1998Review
- The Dictyostelium MAP kinase kinase DdMEK1 regulates chemotaxis and is essential for chemoattractant-mediated activation of guanylyl cyclase.The EMBO journal · 1997Article
- Dual role of cAMP during Dictyostelium development.Experientia · 1995Review
- Expression of cAMP-dependent protein kinase in prespore cells is sufficient to induce spore cell differentiation in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Identification and functional analysis of a developmentally regulated extracellular signal-regulated kinase gene in Dictyostelium discoideum.Molecular and cellular biology · 1994Article
- Developmental decisions in Dictyostelium discoideum.Microbiological reviews · 1994Review
- Activation of the prespore and spore cell pathway of Dictyostelium differentiation by cAMP-dependent protein kinase and evidence for its upstream regulation by ammonia.The EMBO journal · 1993Article
- Use of a transactive regulatory mutant of Dictyostelium discoideum in a eucaryotic expression system.Nucleic acids research · 1993Article
- Transcriptional regulation by folate: inducible gene expression in Dictyostelium transformants during growth and early development.Nucleic acids research · 1992Article
- DdPK3, which plays essential roles during Dictyostelium development, encodes the catalytic subunit of cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Regulation of Cl- transport in T84 cell clones expressing a mutant regulatory subunit of cAMP-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Regulation of slug size by the cell adhesion molecule gp80 in Dictyostelium discoideum.Cell regulation · 1990Article
- Conditions that alter intracellular cAMP levels affect expression of the cAMP phosphodiesterase gene in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the aggregation of Dictyostelium discoideum extracellular cAMP is known to act as a chemotractant and as an inducer of cellular differentiation. However, its intracellular role as a second messenger remains obscure. We have constructed a fusion gene consisting of the cDNA encoding the regulatory subunit (R) of the cAMP-dependent protein kinase fused to the promoter and N-terminal-proximal sequences of a Dictyostelium actin gene. Stable transformants, containing multiple copies of this gene, overproduce the R subunit which accumulates prematurely relative to the endogenous protein. These transformants fail to aggregate. Detailed analysis has shown that they are blocked at interphase, the period prior to aggregation, and that they are severely defective in most responses to cAMP including the induction of gene expression. Our observations suggest that intracellular cAMP acts, presumably by activation of the catalytic subunit of the cAMP-dependent protein kinase, to facilitate early development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.