ArticleInternational journal of biological sciences2014
The insect ecdysone receptor is a good potential target for RNAi-based pest control.
Article in International journal of biological sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 100 citations in OpenAlex.
- RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential.Plants (Basel, Switzerland) · 2026Review
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Two-way transcriptome approach for the identification of common gene targets across four insect orders and its validation in Oxycarenus laetus.Scientific reports · 2025Article
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Genome-wide selection of potential target candidates for RNAi against Nilaparvata lugens.BMC genomics · 2024Article
- Functional Analysis of CPSF30 inInsects · 2024Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Resistance Mechanism ofInsects · 2024Article
- Article
- The ecdysone receptor regulates several key physiological factors in Anopheles funestus.Malaria journal · 2022Article
- Application progress of plant-mediated RNAi in pest control.Frontiers in bioengineering and biotechnology · 2022Review
- Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides.3 Biotech · 2021Review
- RNA interference and crop protection against biotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Effectiveness of orally-delivered double-stranded RNA on gene silencing in the stinkbug Plautia stali.PloS one · 2021Article
- Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the WhiteflyInsects · 2020Review
- Advances in exogenous RNA delivery techniques for RNAi-mediated pest control.Molecular biology reports · 2020Review
- RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti.Parasites & vectors · 2019Article
- Transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say).Transgenic research · 2019Article
- Plant-Mediated RNAi for ControllingFrontiers in plant science · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) has great potential for use in insect pest control. However, some significant challenges must be overcome before RNAi-based pest control can become a reality. One challenge is the proper selection of a good target gene for RNAi. Here, we report that the insect ecdysone receptor (EcR) is a good potential target for RNAi-based pest control in the brown planthopper Nilaparvata lugens, a serious insect pest of rice plants. We demonstrated that the use of a 360 bp fragment (NlEcR-c) that is common between NlEcR-A and NlEcR-B for feeding RNAi experiments significantly decreased the relative mRNA expression levels of NlEcR compared with those in the dsGFP control. Feeding RNAi also resulted in a significant reduction in the number of offspring per pair of N. lugens. Consequently, a transgenic rice line expressing NlEcR dsRNA was constructed by Agrobacterium- mediated transformation. The results of qRT-PCR showed that the total copy number of the target gene in all transgenic rice lines was 2. Northern blot analysis showed that the small RNA of the hairpin dsNlEcR-c was successfully expressed in the transgenic rice lines. After newly hatched nymphs of N. lugens fed on the transgenic rice lines, effective RNAi was observed. The NlEcR expression levels in all lines examined were decreased significantly compared with the control. In all lines, the survival rate of the nymphs was nearly 90%, and the average number of offspring per pair in the treated groups was significantly less than that observed in the control, with a decrease of 44.18-66.27%. These findings support an RNAi-based pest control strategy and are also important for the management of rice insect pests.
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