ArticleScience signaling2014
Npr2 inhibits TORC1 to prevent inappropriate utilization of glutamine for biosynthesis of nitrogen-containing metabolites.
Article in Science signaling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Mechanisms and rationales of SAM homeostasis.Trends in biochemical sciences · 2025Review
- Mitophagy in yeast: known unknowns and unknown unknowns.The Biochemical journal · 2023Review
- Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae.Bioresources and bioprocessing · 2023Article
- Article
- Ammonia promotes the proliferation of bone marrow-derived mesenchymal stem cells by regulating the Akt/mTOR/S6k pathway.Bone research · 2022Article
- Increased glycine contributes to synaptic dysfunction and early mortality in Nprl2 seizure model.iScience · 2022Article
- Effects of abolishing Whi2 on the proteome and nitrogen catabolite repression-sensitive protein production.G3 (Bethesda, Md.) · 2022Article
- SEA and GATOR 10 Years Later.Cells · 2021Review
- Methylated PP2A stabilizes Gcn4 to enable a methionine-induced anabolic program.The Journal of biological chemistry · 2020Article
- S-adenosylmethionine: A metabolite critical to the regulation of autophagy.Cell proliferation · 2020Review
- Phylogenetic debugging of a complete human biosynthetic pathway transplanted into yeast.Nucleic acids research · 2020Article
- Anabolic SIRT4 Exerts Retrograde Control over TORC1 Signaling by Glutamine Sparing in the Mitochondria.Molecular and cellular biology · 2020Article
- Article
- Article
- The SEACIT complex is involved in the maintenance of vacuole-mitochondria contact sites and controls mitophagy.Cellular and molecular life sciences : CMLS · 2019Article
- Methionine at the Heart of Anabolism and Signaling: Perspectives From Budding Yeast.Frontiers in microbiology · 2019Review
- Methionine coordinates a hierarchically organized anabolic program enabling proliferation.Molecular biology of the cell · 2018Article
- Loss of a Negative Regulator of mTORC1 Induces Aerobic Glycolysis and Altered Fiber Composition in Skeletal Muscle.Cell reports · 2018Article
- Metabolic Networks Influencing Skeletal Muscle Fiber Composition.Frontiers in cell and developmental biology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Cells must be capable of switching between growth and autophagy in unpredictable nutrient environments. The conserved Npr2 protein complex (comprising Iml1, Npr2, and Npr3; also called SEACIT) inhibits target of rapamycin complex 1 (TORC1) kinase signaling, which inhibits autophagy in nutrient-rich conditions. In yeast cultured in media with nutrient limitations that promote autophagy and inhibit growth, loss of Npr2 enables cells to bypass autophagy and proliferate. We determined that Npr2-deficient yeast had a metabolic state distinct from that of wild-type yeast when grown in minimal media containing ammonium as a nitrogen source and a nonfermentable carbon source (lactate). Unlike wild-type yeast, which accumulated glutamine, Npr2-deficient yeast metabolized glutamine into nitrogen-containing metabolites and maintained a high concentration of S-adenosyl methionine (SAM). Moreover, in wild-type yeast grown in these nutrient-limited conditions, supplementation with methionine stimulated glutamine consumption for synthesis of nitrogenous metabolites, demonstrating integration of a sulfur-containing amino acid cue and nitrogen utilization. These data revealed the metabolic basis by which the Npr2 complex regulates cellular homeostasis and demonstrated a key function for TORC1 in regulating the synthesis and utilization of glutamine as a nitrogen source.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.