ArticleParasites & vectors2014
Eilat virus displays a narrow mosquito vector range.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Pooled it
- Evaluating the Role ofViruses · 2026Review
- Insect-specific virus platforms for arbovirus vaccine development.Frontiers in immunology · 2025Review
- Synthetic recovery of Yada Yada virus expands insect-specific alphavirus knowledge and facilitates production of chimeric viruses.Npj viruses · 2024Article
- Review
- The infection kinetics and transmission potential of two Guaico Culex viruses in Culex quinquefasciatus mosquitoes.Virologica Sinica · 2024Article
- Article
- A Systematic Review on the Viruses ofTropical medicine and infectious disease · 2023Review
- Culex tarsalis Is a Competent Host of the Insect-Specific Alphavirus Eilat Virus (EILV).Journal of virology · 2023Article
- Article
- A Productive Expression Platform Derived from Host-Restricted Eilat Virus: Its Extensive Validation and Novel Strategy.Viruses · 2021Article
- Symbiotic Interactions Between Mosquitoes and Mosquito Viruses.Frontiers in cellular and infection microbiology · 2021Review
- Superinfection Exclusion in Mosquitoes and Its Potential as an Arbovirus Control Strategy.Viruses · 2020Review
- Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses.PLoS neglected tropical diseases · 2020Article
- Review
- Anopheles gambiae densovirus (AgDNV) negatively affects Mayaro virus infection in Anopheles gambiae cells and mosquitoes.Parasites & vectors · 2020Article
- Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal.Emerging microbes & infections · 2020Article
- Mosquito-Specific Viruses-Transmission and Interaction.Viruses · 2019Review
- Vector Competence: What Has Zika Virus Taught Us?Viruses · 2019Review
- Aedes aegypti vector competence studies: A review.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMost alphaviruses are arthropod-borne and utilize mosquitoes as vectors for transmission to susceptible vertebrate hosts. This ability to infect both mosquitoes and vertebrates is essential for maintenance of most alphaviruses in nature. A recently characterized alphavirus, Eilat virus (EILV), isolated from a pool of Anopheles coustani s.I. is unable to replicate in vertebrate cell lines. The EILV host range restriction occurs at both attachment/entry as well as genomic RNA replication levels. Here we investigated the mosquito vector range of EILV in species encompassing three genera that are responsible for maintenance of other alphaviruses in nature.
methodsSusceptibility studies were performed in four mosquito species: Aedes albopictus, A. aegypti, Anopheles gambiae, and Culex quinquefasciatus via intrathoracic and oral routes utilizing EILV and EILV expressing red fluorescent protein (-eRFP) clones. EILV-eRFP was injected at 10(7) PFU/mL to visualize replication in various mosquito organs at 7 days post-infection. Mosquitoes were also injected with EILV at 10(4)-10(1) PFU/mosquito and virus replication was measured via plaque assays at day 7 post-infection. Lastly, mosquitoes were provided bloodmeals containing EILV-eRFP at doses of 10(9), 10(7), 10(5) PFU/mL, and infection and dissemination rates were determined at 14 days post-infection.
resultsAll four species were susceptible via the intrathoracic route; however, replication was 10-100 fold less than typical for most alphaviruses, and infection was limited to midgut-associated muscle tissue and salivary glands. A. albopictus was refractory to oral infection, while A. gambiae and C. quinquefasciatus were susceptible only at 10(9) PFU/mL dose. In contrast, A. aegypti was susceptible at both 10(9) and 10(7) PFU/mL doses, with body infection rates of 78% and 63%, and dissemination rates of 26% and 8%, respectively.
conclusionsThe exclusion of vertebrates in its maintenance cycle may have facilitated the adaptation of EILV to a single mosquito host. As a consequence, EILV displays a narrow vector range in mosquito species responsible for the maintenance of other alphaviruses in nature.
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