ArticleAngewandte Chemie (International ed. in English)2015
Tracking surface glycans on live cancer cells with single-molecule sensitivity.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- A Multiscale Computational Architecture to Study Signaling Dynamics at Cell-Cell Interfaces.bioRxiv : the preprint server for biology · 2026Article
- In Situ Glycan Analysis and Editing in Living Systems.JACS Au · 2024Review
- Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer.Chemical science · 2022Article
- Azide-based bioorthogonal chemistry: Reactions and its advances in cellular and biomolecular imaging.Biophysics reviews · 2021Review
- Using unnatural amino acids to selectively label proteins for cellular imaging: a cell biologist viewpoint.The FEBS journal · 2021Review
- Click-ExM enables expansion microscopy for all biomolecules.Nature methods · 2021Article
- Fluorophore-Assisted Click Chemistry through Copper(I) Complexation.Biomolecules · 2020Article
- Modeling membrane nanotube morphology: the role of heterogeneity in composition and material properties.Scientific reports · 2020Article
- The Emerging Role of the Mammalian Glycocalyx in Functional Membrane Organization and Immune System Regulation.Frontiers in cell and developmental biology · 2020Review
- Fluorometric visualization of mucin 1 glycans on cell surfaces based on rolling-mediated cascade amplification and CdTe quantum dots.Mikrochimica acta · 2019Article
- Quantitative Super-Resolution Microscopy of the Mammalian Glycocalyx.Developmental cell · 2019Article
- Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Simultaneous Multicolor Single-Molecule Tracking with Single-Laser Excitation via Spectral Imaging.Biophysical journal · 2018Article
- General principles of binding between cell surface receptors and multi-specific ligands: A computational study.PLoS computational biology · 2017Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Visualizing glycans on single cells and tissues-Visualizing glycans on single cells and tissues.Current opinion in chemical biology · 2017Review
- A flow cytometry assay to quantify intercellular exchange of membrane components.Chemical science · 2017Article
- Modern Synthetic Avenues for the Preparation of Functional Fluorophores.Angewandte Chemie (International ed. in English) · 2017Review
- Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling.ACS chemical biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Using a combination of metabolically labeled glycans, a bioorthogonal copper(I)-catalyzed azide-alkyne cycloaddition, and the controlled bleaching of fluorescent probes conjugated to azide- or alkyne-tagged glycans, a sufficiently low spatial density of dye-labeled glycans was achieved, enabling dynamic single-molecule tracking and super-resolution imaging of N-linked sialic acids and O-linked N-acetyl galactosamine (GalNAc) on the membrane of live cells. Analysis of the trajectories of these dye-labeled glycans in mammary cancer cells revealed constrained diffusion of both N- and O-linked glycans, which was interpreted as reflecting the mobility of the glycan rather than to be caused by transient immobilization owing to spatial inhomogeneities on the plasma membrane. Stochastic optical reconstruction microscopy (STORM) imaging revealed the structure of dynamic membrane nanotubes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.