ArticleMicrobiologyOpen2015
The evolution of Jen3 proteins and their role in dicarboxylic acid transport in Yarrowia.
Article in MicrobiologyOpen, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production inSynthetic and systems biotechnology · 2026Article
- Transcriptomics-based analysis of acetate and propionate transport and metabolism in Yarrowia lipolytica.Biotechnology for biofuels and bioproducts · 2025Article
- Boosting succinic acid production of Yarrowia lipolytica at low pH through enhancing product tolerance and glucose metabolism.Microbial cell factories · 2024Article
- Uncovering Novel Plasma Membrane Carboxylate Transporters in the YeastJournal of fungi (Basel, Switzerland) · 2022Article
- Characterization of JEN family carboxylate transporters from the acid-tolerant yeast Pichia kudriavzevii and their applications in succinic acid production.Microbial biotechnology · 2021Article
- Carboxylic Acid Transporters inmBio · 2020Review
- Elucidating the Role and Regulation of a Lactate Permease as Lactate Transporter in Bacillus coagulans DSM1.Applied and environmental microbiology · 2019Article
- Ancestral Function and Diversification of a Horizontally Acquired Oomycete Carboxylic Acid Transporter.Molecular biology and evolution · 2018Article
- Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica.Microbial cell factories · 2017Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Jen proteins in yeast are involved in the uptake of mono/dicarboxylic acids. The Jen1 subfamily transports lactate and pyruvate, while the Jen2 subfamily transports fumarate, malate, and succinate. Yarrowia lipolytica has six JEN genes: YALI0B19470g, YALI0C15488g, YALI0C21406g, YALI0D20108g, YALI0D24607g, and YALI0E32901g. Through phylogenetic analyses, we found that these genes represent a new subfamily, Jen3 and that these three Jen subfamilies derivate from three putative ancestral genes. Reverse transcription-PCR. revealed that only four YLJEN genes are expressed and they are upregulated in the presence of lactate, pyruvate, fumarate, malate, and/or succinate, suggesting that they are able to transport these substrates. Analysis of deletion mutant strains revealed that Jen3 subfamily proteins transport fumarate, malate, and succinate. We found evidence that YALI0C15488 encodes the main transporter because its deletion was sufficient to strongly reduce or suppress growth in media containing fumarate, malate, or succinate. It appears that the other YLJEN genes play a minor role, with the exception of YALI0E32901g, which is important for malate uptake. However, the overexpression of each YLJEN gene in the sextuple-deletion mutant strain ΔYLjen1-6 revealed that all six genes are functional and have evolved to transport different substrates with varying degrees of efficacy. In addition, we found that YALI0E32901p transported succinate more efficiently in the presence of lactate or fumarate.
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