ArticleNucleic acids research2015
NERF encodes a RING E3 ligase important for drought resistance and enhances the expression of its antisense gene NFYA5 in Arabidopsis.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 52 citations in OpenAlex.
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- Silencing an E3 Ubiquitin Ligase GeneInternational journal of molecular sciences · 2021Article
- The chloroplast-associated protein degradation pathway controls chromoplast development and fruit ripening in tomato.Nature plants · 2021Article
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- Insights Into Genetic and Molecular Elements for Transgenic Crop Development.Frontiers in plant science · 2020Article
- Research Progress on Plant RING-Finger Proteins.Genes · 2019Review
- Control of Cognate Sense mRNA Translation by cis-Natural Antisense RNAs.Plant physiology · 2019Article
- Abiotic stress miRNomes in the Triticeae.Functional & integrative genomics · 2017Review
- Pepper CaREL1, a ubiquitin E3 ligase, regulates drought tolerance via the ABA-signalling pathway.Scientific reports · 2017Article
- Genome-wide identification, evolution and expression analysis of RING finger protein genes in Brassica rapa.Scientific reports · 2017Article
- Genomic Selection for Drought Tolerance Using Genome-Wide SNPs in Maize.Frontiers in plant science · 2017Article
- Cesium Toxicity Alters MicroRNA Processing and AGO1 Expressions in Arabidopsis thaliana.PloS one · 2015Article
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4 authors at 2 institutions in 1 country.
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Abstract
NFYA5 is an important drought-stress inducible transcription factor gene that is targeted by miR169 in Arabidopsis. We show here that the cis-natural antisense transcript gene of NFYA5, NFYA5 Enhancing RING FINGER (NERF), can produce siRNAs from their overlapping region (OR) and affect NFYA5 transcripts by functioning together with miR169. The NERF protein functions as an E3 ligase for ubiquitination. Overexpression of NERF or OR cDNA leads to siRNANERF accumulation, miR169 repression, and NFYA5 transcript enhancement; knock-down of NERF transcripts by an artificial miRNA enhances miR169 abundance and reduces NFYA5 transcripts. Overexpression of NFYA5 does not affect the NERF mRNA level. Deep sequencing of the small RNA library from 35S::OR plants identifies 960 sequences representing 323 unique siRNAs that originate from OR; the sequences of some siRNANERF are similar/complementary to those of miR169. Overexpression of the 195- to 280-bp OR cDNA-containing siRNAs similar/complementary to miR169 also leads to the accumulation of NFYA5 transcripts. Analysis of NERF knock-down plants and NERF overexpression lines showed that, like NFYA5, NERF is important for controlling stomatal aperture and drought resistance. This regulatory model might apply to other natural antisense transcripts with positively correlated expression patterns.
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