ArticlePloS one2014
Epithelial cells are active participants in vocal fold wound healing: an in vivo animal model of injury.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Laryngeal Basal Cell Response to Cigarette Smoke and E-Cigarette Aerosol Exposures.The Laryngoscope · 2026Article
- Multi-timescale finite element modeling of vocal fold edema progression due to phonotrauma.The Journal of the Acoustical Society of America · 2026Article
- Preclinical Vocal Fold and Airway Injury Models: A Scoping Review.Laryngoscope investigative otolaryngology · 2025Review
- Controlled decorin delivery from injectable microgels promotes scarless vocal fold repair.bioRxiv : the preprint server for biology · 2025Article
- Establishing a Mouse Model of Surgical Vocal Fold Injury.The Laryngoscope · 2025Article
- Effect of fenugreek oil on healing of experimentally induced buccal mucosal ulcer by immunohistochemical evaluation of Ki-67 expression.Cell biochemistry and biophysics · 2024Article
- Method for Collecting Single Epithelial Cells from the Mouse Larynx.The Laryngoscope · 2024Article
- Article
- The role of systemic dehydration in vocal fold healing: Preliminary findings.Laryngoscope investigative otolaryngology · 2022Article
- Tissue Engineering as a Promising Treatment for Glottic Insufficiency: A Review on Biomolecules and Cell-Laden Hydrogel.Biomedicines · 2022Review
- Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling.Disease models & mechanisms · 2022Article
- Topical phenytoin administration accelerates the healing of acetic acid-induced colitis in rats: evaluation of transforming growth factor-beta, platelet-derived growth factor, and vascular endothelial growth factor.Inflammopharmacology · 2022Article
- Singer's Nodules: Investigating the Etiopathogenetic Markers Progressing Their Pathogenesis and Clinical Manifestations.Biology · 2021Article
- Mainstream Cigarette Smoke Impacts the Mouse Vocal Fold Epithelium and Mucus Barrier.The Laryngoscope · 2021Observational
- Hydrogel-Supported, Engineered Model of Vocal Fold Epithelium.ACS biomaterials science & engineering · 2021Article
- Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Short-term whole body cigarette smoke exposure induces regional differences in cellular response in the mouse larynx.Toxicology reports · 2021Article
- Protein Substrate Alters Cell Physiology in Primary Culture of Vocal Fold Epithelial Cells.Cells, tissues, organs · 2021Article
- Transcriptomic and Morphological Analysis of Cells Derived from Porcine Buccal Mucosa-Studies on an In Vitro Model.Animals : an open access journal from MDPI · 2020Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Vocal fold epithelial cells likely play an important, yet currently poorly defined, role in healing following injury, irritation and inflammation. In the present study, we sought to identify a possible role for growth factors, epidermal growth factor (EGF) and transforming growth factor-beta 1 (TGFβ1), in epithelial regeneration during wound healing as a necessary first step for uncovering potential signaling mechanisms of vocal fold wound repair and remodeling. Using a rat model, we created unilateral vocal fold injuries and examined the timeline for epithelial healing and regeneration during early and late stages of wound healing using immunohistochemistry (IHC). We observed time-dependent secretion of the proliferation marker, ki67, growth factors EGF and TGFβ1, as well as activation of the EGF receptor (EGFR), in regenerating epithelium during the acute phase of injury. Ki67, growth factor, and EGFR expression peaked at day 3 post-injury. Presence of cytoplasmic and intercellular EGF and TGFβ1 staining occurred up to 5 days post-injury, consistent with a role for epithelial cells in synthesizing and secreting these growth factors. To confirm that epithelial cells contributed to the cytokine secretion, we examined epithelial cell growth factor secretion in vitro using polymerase chain reaction (PCR). Cultured pig vocal fold epithelial cells expressed both EGF and TGFβ1. Our in vivo and in vitro findings indicate that epithelial cells are active participants in the wound healing process. The exact mechanisms underlying their roles in autocrine and paracrine signaling guiding wound healing await study in a controlled, in vitro environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.