ArticleJournal of bacteriology2015
Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution.
Article in Journal of bacteriology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods.Cell reports methods · 2026Article
- Genomic and functional dissection of natural transformation-related genes inMicrobiology spectrum · 2026Article
- Bacteria as living biosensors for DNA.Nature reviews bioengineering · 2026Article
- Mutations in filamentous bacteriophages spark eco-evolutionary feedbacks in Pseudomonas aeruginosa.The ISME journal · 2026Article
- Article
- Genome evolution ofApplied and environmental microbiology · 2025Article
- Naturally competent bacteria and their genetic parasites-a battle for control over horizontal gene transfer?FEMS microbiology reviews · 2025Review
- Experimental evolution under different nutritional conditions changes the genomic architecture and virulence of Acinetobacter baumannii.Communications biology · 2024Article
- Evolution recovers the fitness of Acinetobacter baylyi strains with large deletions through mutations in deletion-specific targets and global post-transcriptional regulators.PLoS genetics · 2024Article
- Reduced social function in experimentally evolvedProceedings. Biological sciences · 2023Article
- Testing for the fitness benefits of natural transformation during community-embedded evolution.Microbiology (Reading, England) · 2023Article
- Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae.mBio · 2023Article
- Horizontal Gene Transfer, Fitness Costs and Mobility Shape the Spread of Antibiotic Resistance Genes into Experimental Populations of Acinetobacter Baylyi.Molecular biology and evolution · 2023Article
- Characterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.Applied and environmental microbiology · 2022Article
- One-Day Construction of Multiplex Arrays to Harness Natural CRISPR-Cas Systems.ACS synthetic biology · 2020Article
- Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining.Nucleic acids research · 2020Article
- Genome alterations associated with improved transformation efficiency in Lactobacillus reuteri.Microbial cell factories · 2018Article
- Microfluidics-Based Analysis of Contact-dependent Bacterial Interactions.Bio-protocol · 2018Article
- Basic Characterization of Natural Transformation in a Highly TransformableFrontiers in cellular and infection microbiology · 2018Article
- Reduced Mutation Rate and Increased Transformability of Transposon-Free Acinetobacter baylyi ADP1-ISx.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic biology because it is naturally transformable. To examine the genetic reliability of this desirable trait and to understand the potential stability of other engineered capabilities, we propagated ADP1 for 1,000 generations of growth in rich nutrient broth and analyzed the genetic changes that evolved by whole-genome sequencing. Substantially reduced transformability and increased cellular aggregation evolved during the experiment. New insertions of IS1236 transposable elements and IS1236-mediated deletions led to these phenotypes in most cases and were common overall among the selected mutations. We also observed a 49-kb deletion of a prophage region that removed an integration site, which has been used for genome engineering, from every evolved genome. The comparatively low rates of these three classes of mutations in lineages that were propagated with reduced selection for 7,500 generations indicate that they increase ADP1 fitness under common laboratory growth conditions. Our results suggest that eliminating transposable elements and other genetic failure modes that affect key organismal traits is essential for improving the reliability of metabolic engineering and genome editing in undomesticated microbial hosts, such as Acinetobacter baylyi ADP1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.