ArticleMolecular plant pathology2015
Development of plants resistant to tomato geminiviruses using artificial trans-acting small interfering RNA.
Article in Molecular plant pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- amiR414e-mediated silencing confers resistance to Cotton leaf curl Multan virus in cotton.Molecular biology reports · 2026Article
- syn-tasiRnas targeting the coat protein of potato virus Y confer antiviral resistance inPlant signaling & behavior · 2024Article
- Advances in understanding plant-pathogen interactions: insights from tomato as a model system.Virusdisease · 2024Review
- Exogenous Application of dsRNA for Protection against Tomato Leaf Curl New Delhi Virus.Viruses · 2024Article
- Identification of pathogenicity determinants in ToLCNDV and their RNAi-based knockdown for disease management inFrontiers in microbiology · 2024Article
- Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions.Stress biology · 2022Review
- Plant responses to geminivirus infection: guardians of the plant immunity.Virology journal · 2021Review
- The role of miRNA in plant-virus interaction: a review.Molecular biology reports · 2021Review
- Secondary siRNAs in Plants: Biosynthesis, Various Functions, and Applications in Virology.Frontiers in plant science · 2021Review
- Clay nanosheet-mediated delivery of recombinant plasmids expressing artificial miRNAs via leaf spray to prevent infection by plant DNA viruses.Horticulture research · 2020Article
- Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants.Plants (Basel, Switzerland) · 2020Review
- RNAi-Mediated Resistance Against Viruses in Perennial Fruit Plants.Plants (Basel, Switzerland) · 2019Review
- Secondary Small Interfering RNA-Based Silencing Tools in Plants: An Update.Frontiers in plant science · 2019Article
- Multiplexed editing of a begomovirus genome restricts escape mutant formation and disease development.PloS one · 2019Article
- Tomato Leaf Curl New Delhi Virus: An Emerging Virus Complex Threatening Vegetable and Fiber Crops.Viruses · 2017Review
- Review
- Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection.Molecular plant pathology · 2017Article
- The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead.Frontiers in plant science · 2017Review
- Genetically modified (GM) crops: milestones and new advances in crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi), a conserved RNA-mediated gene regulatory mechanism in eukaryotes, plays an important role in plant growth and development, and as an antiviral defence system in plants. As a counter-strategy, plant viruses encode RNAi suppressors to suppress the RNAi pathways and consequently down-regulate plant defence. In geminiviruses, the proteins AC2, AC4 and AV2 are known to act as RNAi suppressors. In this study, we have designed a gene silencing vector using the features of trans-acting small interfering RNA (tasiRNA), which is simple and can be used to target multiple genes at a time employing a single-step cloning procedure. This vector was used to target two RNAi suppressor proteins (AC2 and AC4) of the geminivirus, Tomato leaf curl New Delhi virus (ToLCNDV). The vector containing fragments of ToLCNDV AC2 and AC4 genes, on agro-infiltration, produced copious quantities of AC2 and AC4 specific siRNA in both tobacco and tomato plants. On challenge inoculation of the agro-infiltrated plants with ToLCNDV, most plants showed an absence of symptoms and low accumulation of viral DNA. Transgenic tobacco plants were raised using the AC2 and AC4 tasiRNA-generating constructs, and T1 plants, obtained from the primary transgenic plants, were tested for resistance separately against ToLCNDV and Tomato leaf curl Gujarat virus. Most plants showed an absence of symptoms and low accumulation of the corresponding viruses, the resistance being generally proportional to the amounts of siRNA produced against AC2 and AC4 genes. This is the first report of the use of artificial tasiRNA to generate resistance against an important plant virus.
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