ArticlePlant molecular biology2015
Biofortification of rice with lysine using endogenous histones.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Lysine-rich rice enhanced muscle growth and development in young rats.European journal of nutrition · 2025Article
- Local Sources of Protein in Low- and Middle-Income Countries: How to Improve the Protein Quality?Current developments in nutrition · 2024Article
- Review
- Combined Hybridization and Evaluation of High-Lysine Rice: Nutritional and Physicochemical Qualities and Field Performance.International journal of molecular sciences · 2022Article
- Amino Acids in Rice Grains and Their Regulation by Polyamines and Phytohormones.Plants (Basel, Switzerland) · 2022Review
- Genes and Their Molecular Functions Determining Seed Structure, Components, and Quality of Rice.Rice (New York, N.Y.) · 2022Review
- Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.Transgenic research · 2022Article
- Extreme Low-Temperature Stress Affects Nutritional Quality of Amino Acids in Rice.Frontiers in plant science · 2022Article
- Lysine biofortification in rice by modulating feedback inhibition of aspartate kinase and dihydrodipicolinate synthase.Plant biotechnology journal · 2021Article
- Biofortification of Cereals and Pulses Using New Breeding Techniques: Current and Future Perspectives.Frontiers in nutrition · 2021Review
- Nutrition and antioxidant profiling in the unpolished and polished grains of eleven indigenous aromatic rice cultivars.3 Biotech · 2020Article
- Improving nutritional quality of rice for human health.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Targeting Nitrogen Metabolism and Transport Processes to Improve Plant Nitrogen Use Efficiency.Frontiers in plant science · 2020Review
- Connections Between Amino Acid Metabolisms in Plants: Lysine as an Example.Frontiers in plant science · 2020Review
- Genetic Manipulation for Improved Nutritional Quality in Rice.Frontiers in genetics · 2020Review
- Improved growth performance, food efficiency, and lysine availability in growing rats fed with lysine-biofortified rice.Scientific reports · 2017Article
- Production of Marker-free Transgenic Rice (Iranian journal of biotechnology · 2017Article
- Improving protein content and quality by over-expressing artificially synthetic fusion proteins with high lysine and threonine constituent in rice plants.Scientific reports · 2016Article
- Pyramiding and evaluation of both a foreignBreeding science · 2016Article
- Biofortification of rice with the essential amino acid lysine: molecular characterization, nutritional evaluation, and field performance.Journal of experimental botany · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice is the most consumed cereal grain in the world, but deficient in the essential amino acid lysine. Therefore, people in developing countries with limited food diversity who rely on rice as their major food source may suffer from malnutrition. Biofortification of stable crops by genetic engineering provides a fast and sustainable method to solve this problem. In this study, two endogenous rice lysine-rich histone proteins, RLRH1 and RLRH2, were over-expressed in rice seeds to achieve lysine biofortification. Their protein sequences passed an allergic sequence-based homology test. Their accumulations in rice seeds were raised to a moderate level by the use of a modified rice glutelin 1 promoter with lowered expression strength to avoid the occurrence of physiological abnormalities like unfolded protein response. The expressed proteins were further targeted to protein storage vacuoles for stable storage using a glutelin 1 signal peptide. The lysine content in the transgenic rice seeds was enhanced by up to 35 %, while other essential amino acids remained balanced, meeting the nutritional standards of the World Health Organization. No obvious unfolded protein response was detected. Different degrees of chalkiness, however, were detected in the transgenic seeds, and were positively correlated with both the levels of accumulated protein and lysine enhancement. This study offered a solution to the lysine deficiency in rice, while at the same time addressing concerns about food safety and physiological abnormalities in biofortified crops.
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