ArticleBiotechnology journal2015
Isothermal microcalorimetry accurately detects bacteria, tumorous microtissues, and parasitic worms in a label-free well-plate assay.
Article in Biotechnology journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Chip Calorimetry for Single-Cell Analysis: Advances, Challenges, and Opportunities.Sensors (Basel, Switzerland) · 2026Review
- Poly(hydroxy-oxazolidone) Thermoplastic Elastomers for Safer, Greener and Customizable Blood-Contacting Medical Devices.Advanced healthcare materials · 2025Article
- Treatment response evaluation in anFrontiers in pediatrics · 2025Article
- The development of ultrasensitive microcalorimeters for bioanalysis and energy balance monitoring.Fundamental research · 2024Review
- Targeting Pseudomonas aeruginosa biofilm with an evolutionary trained bacteriophage cocktail exploiting phage resistance trade-offs.Nature communications · 2024Article
- Exploring the resilience and stability of a defined human gut microbiota consortium: An isothermal microcalorimetric study.MicrobiologyOpen · 2024Article
- Bacterial micro-aggregates as inoculum in animal models of implant-associated infections.Biofilm · 2024Article
- Proof of concept: real-time viability and metabolic profiling of probiotics with isothermal microcalorimetry.Frontiers in microbiology · 2024Article
- Selenomonas sputigena acts as a pathobiont mediating spatial structure and biofilm virulence in early childhood caries.Nature communications · 2023Article
- Article
- Dehydration Tolerance in Epidemic versus Nonepidemic MRSA Demonstrated by Isothermal Microcalorimetry.Microbiology spectrum · 2022Article
- Understanding orthopedic infections through a different perspective: Microcalorimetry growth curves.Experimental and therapeutic medicine · 2022Article
- Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.International journal of molecular sciences · 2022Article
- Anatomical Site-Specific Carbohydrate Availability Impacts Streptococcus pneumoniae Virulence and Fitness during Colonization and Disease.Infection and immunity · 2022Article
- Increased Proliferation of Neuroblastoma Cells under Fructose Metabolism Can Be Measured by Isothermal Microcalorimetry.Children (Basel, Switzerland) · 2021Article
- Metastatic Esophageal Carcinoma Cells Exhibit Reduced Adhesion Strength and Enhanced Thermogenesis.Cells · 2021Article
- Avoiding transduction-induced heating in suspended microchannel resonators using piezoelectricity.Microsystems & nanoengineering · 2021Article
- Schistosomiasis Drug Discovery in the Era of Automation and Artificial Intelligence.Frontiers in immunology · 2021Review
- Rapid culture-based detection of Legionella pneumophila using isothermal microcalorimetry with an improved evaluation method.Microbial biotechnology · 2020Article
- Personalized Treatment Response Assessment for Rare Childhood Tumors Using Microcalorimetry-Exemplified by Use of Carbonic Anhydrase IX and Aquaporin 1 Inhibitors.International journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isothermal microcalorimetry is a label-free assay that allows monitoring of enzymatic and metabolic activities. The technique has strengths, but most instruments have a low throughput, which has limited their use for bioassays. Here, an isothermal microcalorimeter, equipped with a vessel holder similar to a 48-well plate, was used. The increased throughput of this microcalorimeter makes it valuable for biomedical and pharmaceutical applications. Our results show that the sensitivity of the instrument allows the detection of 3 × 10(4) bacteria per vial. Growth of P. mirabilis in Luria Broth medium was detected between 2 and 9 h with decreasing inoculum. The culture released 2.1J with a maximum thermal power of 76 μW. The growth rate calculated using calorimetric and spectrophotometric data were 0.60 and 0.57 h(-1) , respectively. Additional insight on protease activities of P. mirabilis matching the last peak in heat production could be gathered as well. Growth of tumor microtissues releasing a maximum thermal power of 2.1 μW was also monitored and corresponds to a diameter increase of the microtissues from ca. 100 to 428 μm. This opens new research avenues in cancer research, diagnostics, and development of new antitumor drugs. For parasitic worms, the technique allows assessment of parasite survival using motor and metabolic activities even with a single worm.
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