ArticlePlant physiology2015
Genome-based metabolic mapping and 13C flux analysis reveal systematic properties of an oleaginous microalga Chlorella protothecoides.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 106 citations in OpenAlex.
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- Harnessing Artificial Intelligence to Revolutionize Microalgae Biotechnology: Unlocking Sustainable Solutions for High-Value Compounds and Carbon Neutrality.Marine drugs · 2025Review
- Unraveling metabolism underpinning biomass composition shift inFrontiers in plant science · 2025Article
- Central transcriptional regulator controls photosynthetic growth and carbon storage in response to high light.Nature communications · 2024Article
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- Re-Programing Glucose Catabolism in the MicroalgaBiomolecules · 2022Article
- Multi-Objective Optimization of Microalgae Metabolism: An Evolutive Algorithm Based on FBA.Metabolites · 2022Article
- The carbon partitioning of glucose and DIC in mixotrophic, heterotrophic and photoautotrophic cultures of Tetraselmis suecica.Biotechnology letters · 2021Article
- Systematic identification and elimination of flux bottlenecks in the aldehyde production pathway of Synechococcus elongatus PCC 7942.Metabolic engineering · 2020Article
- Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina.BMC bioinformatics · 2020Article
- Parallel isotope differential modeling for instationary 13C fluxomics at the genome scale.Biotechnology for biofuels · 2020Article
- Bioengineering of Microalgae: Recent Advances, Perspectives, and Regulatory Challenges for Industrial Application.Frontiers in bioengineering and biotechnology · 2020Review
- Inhibition of glucose assimilation inBiotechnology for biofuels · 2020Article
- Article
- A kinetic metabolic study of lipid production in Chlorella protothecoides under heterotrophic condition.Microbial cell factories · 2019Article
- Genome-Scale Fluxome ofPlant physiology · 2019Article
- Label-free quantitative proteomics to investigate the response of strawberry fruit after controlled ozone treatment.RSC advances · 2019Article
- Utilizing genome-scale models to optimize nutrient supply for sustained algal growth and lipid productivity.NPJ systems biology and applications · 2019Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integrated and genome-based flux balance analysis, metabolomics, and (13)C-label profiling of phototrophic and heterotrophic metabolism in Chlorella protothecoides, an oleaginous green alga for biofuel. The green alga Chlorella protothecoides, capable of autotrophic and heterotrophic growth with rapid lipid synthesis, is a promising candidate for biofuel production. Based on the newly available genome knowledge of the alga, we reconstructed the compartmentalized metabolic network consisting of 272 metabolic reactions, 270 enzymes, and 461 encoding genes and simulated the growth in different cultivation conditions with flux balance analysis. Phenotype-phase plane analysis shows conditions achieving theoretical maximum of the biomass and corresponding fatty acid-producing rate for phototrophic cells (the ratio of photon uptake rate to CO2 uptake rate equals 8.4) and heterotrophic ones (the glucose uptake rate to O2 consumption rate reaches 2.4), respectively. Isotope-assisted liquid chromatography-mass spectrometry/mass spectrometry reveals higher metabolite concentrations in the glycolytic pathway and the tricarboxylic acid cycle in heterotrophic cells compared with autotrophic cells. We also observed enhanced levels of ATP, nicotinamide adenine dinucleotide (phosphate), reduced, acetyl-Coenzyme A, and malonyl-Coenzyme A in heterotrophic cells consistently, consistent with a strong activity of lipid synthesis. To profile the flux map in experimental conditions, we applied nonstationary (13)C metabolic flux analysis as a complementing strategy to flux balance analysis. The result reveals negligible photorespiratory fluxes and a metabolically low active tricarboxylic acid cycle in phototrophic C. protothecoides. In comparison, high throughput of amphibolic reactions and the tricarboxylic acid cycle with no glyoxylate shunt activities were measured for heterotrophic cells. Taken together, the metabolic network modeling assisted by experimental metabolomics and (13)C labeling better our understanding on global metabolism of oleaginous alga, paving the way to the systematic engineering of the microalga for biofuel production.
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