ArticleCellular and molecular life sciences : CMLS2015
MANF silencing, immunity induction or autophagy trigger an unusual cell type in metamorphosing Drosophila brain.
Article in Cellular and molecular life sciences : CMLS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 29 citations in OpenAlex.
- Quality Evaluation Based Simulation Selection (QEBSS) for analysis of conformational ensembles and dynamics of multidomain proteins.Communications chemistry · 2025Article
- The Drosophila adult brain: short overview of structure, function, and resources Graphical Review Paper.Current research in insect science · 2025Review
- Ensheathing glia promote increased lifespan and healthy brain aging.Aging cell · 2023Article
- Review
- On the origin of the functional versatility of macrophages.Frontiers in physiology · 2023Article
- Insights into nervous system repair from the fruit fly.Neuronal signaling · 2022Review
- UPR Responsive GenesFrontiers in cellular neuroscience · 2022Review
- Brain inflammation triggers macrophage invasion across the blood-brain barrier inScience advances · 2021Article
- Mono-macrophage-Derived MANF Alleviates Bacterial Myocarditis by Inhibiting NF-kappaB Activation and Myocardial Inflammation.Inflammation · 2021Article
- Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity inFrontiers in physiology · 2021Article
- Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice.Autophagy · 2020Article
- MANF Promotes Differentiation and Migration of Neural Progenitor Cells with Potential Neural Regenerative Effects in Stroke.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Different Levels of Expression of the Clock Protein PER and the Glial Marker REPO in Ensheathing and Astrocyte-Like Glia of the Distal Medulla ofFrontiers in physiology · 2018Article
- Role of Glial Immunity in Lifespan Determination: AFrontiers in immunology · 2018Review
- Connecting the nervous and the immune systems in evolution.Communications biology · 2018Article
- An evolutionary perspective on the role of mesencephalic astrocyte-derived neurotrophic factor (MANF): At the crossroads of poriferan innate immune and apoptotic pathways.Biochemistry and biophysics reports · 2017Article
- Downregulation ofFrontiers in neuroscience · 2017Article
- Origins of glial cell populations in the insect nervous system.Current opinion in insect science · 2016Review
- Immune modulation by MANF promotes tissue repair and regenerative success in the retina.Science (New York, N.Y.) · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glia are abundant cells in the brain of animals ranging from flies to humans. They perform conserved functions not only in neural development and wiring, but also in brain homeostasis. Here we show that by manipulating gene expression in glia, a previously unidentified cell type appears in the Drosophila brain during metamorphosis. More specifically, this cell type appears in three contexts: (1) after the induction of either immunity, or (2) autophagy, or (3) by silencing of neurotrophic factor DmMANF in glial cells. We call these cells MANF immunoreactive Cells (MiCs). MiCs are migratory based on their shape, appearance in brain areas where no cell bodies exist and the nuclear localization of dSTAT. They are labeled with a unique set of molecular markers including the conserved neurotrophic factor DmMANF and the transcription factor Zfh1. They possess the nuclearly localized protein Relish, which is the hallmark of immune response activation. They also express the conserved engulfment receptor Draper, therefore indicating that they are potentially phagocytic. Surprisingly, they do not express any of the common glial and neuronal markers. In addition, ultrastructural studies show that MiCs are extremely rich in lysosomes. Our findings reveal critical molecular and functional components of an unusual cell type in the Drosophila brain. We suggest that MiCs resemble macrophages/hemocytes and vertebrate microglia based on their appearance in the brain upon genetically challenged conditions and the expression of molecular markers. Interestingly, macrophages/hemocytes or microglia-like cells have not been reported in the fly nervous system before.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.