ArticleToxicology2015
Multi-walled carbon nanotube-induced gene expression in vitro: concordance with in vivo studies.
Article in Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.Particle and fibre toxicology · 2023Article
- U.S. Federal Agency interests and key considerations for new approach methodologies for nanomaterials.ALTEX · 2022Article
- Carbon Nanotubes as Carriers in Drug Delivery for Non-Small Cell Lung Cancer, Mechanistic Analysis of Their Carcinogenic Potential, Safety Profiling and Identification of Biomarkers.International journal of nanomedicine · 2022Review
- New "Omics" Approaches as Tools to Explore Mechanistic Nanotoxicology.Advances in experimental medicine and biology · 2022Article
- Transcriptome Profile Alterations with Carbon Nanotubes, Quantum Dots, and Silver Nanoparticles: A Review.Genes · 2021Review
- Role of Surface Chemistry in the In Vitro Lung Response to Nanofibrillated Cellulose.Nanomaterials (Basel, Switzerland) · 2021Article
- Pilot deep RNA sequencing of worker blood samples from Singapore printing industry for occupational risk assessment.NanoImpact · 2020Article
- Comparative analysis of lung and blood transcriptomes in mice exposed to multi-walled carbon nanotubes.Toxicology and applied pharmacology · 2020Article
- Integrated Transcriptomics, Metabolomics, and Lipidomics Profiling in Rat Lung, Blood, and Serum for Assessment of Laser Printer-Emitted Nanoparticle Inhalation Exposure-Induced Disease Risks.International journal of molecular sciences · 2019Article
- Multi-Walled Carbon Nanotube-Induced Gene Expression Biomarkers for Medical and Occupational Surveillance.International journal of molecular sciences · 2019Article
- A novel human 3D lung microtissue model for nanoparticle-induced cell-matrix alterations.Particle and fibre toxicology · 2019Article
- Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure.Toxicology reports · 2019Article
- Biological monitoring of workers exposed to engineered nanomaterials.Toxicology letters · 2018Review
- Multi-omics analysis of ten carbon nanomaterials effects highlights cell type specific patterns of molecular regulation and adaptation.NanoImpact · 2018Article
- Substantial modification of the gene expression profile following exposure of macrophages to welding-related nanoparticles.Scientific reports · 2018Article
- Application of bi-clustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials.Data in brief · 2017Article
- Carbon nanotubes in microfluidic lab-on-a-chip technology: current trends and future perspectives.Microfluidics and nanofluidics · 2017Article
- A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry.Scientific reports · 2017Article
- Critical review of the current and future challenges associated with advanced in vitro systems towards the study of nanoparticle (secondary) genotoxicity.Mutagenesis · 2017Review
- Fibrosis biomarkers in workers exposed to MWCNTs.Toxicology and applied pharmacology · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
There is a current interest in reducing the in vivo toxicity testing of nanomaterials in animals by increasing toxicity testing using in vitro cellular assays; however, toxicological results are seldom concordant between in vivo and in vitro models. This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. Additionally, different cellular signaling pathways are activated in response to MWCNT dependent upon culturing conditions. As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.