ArticleThe American journal of tropical medicine and hygiene2015
Recombinase polymerase amplification-based assay to diagnose Giardia in stool samples.
Article in The American journal of tropical medicine and hygiene, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Survey ofTransboundary and emerging diseases · 2025Article
- Recombinase polymerase amplification - lateral flow dipstick for rapid and visual detection ofFrontiers in cellular and infection microbiology · 2024Article
- Development of an LFD-RPA Assay for Rapid Detection ofCurrent issues in molecular biology · 2023Article
- Development and application of recombinase polymerase amplification assay for rapid detection ofParasitology · 2023Article
- Development and Visualization Improvement for the Rapid Detection of Decapod Iridescent Virus 1 (DIV1) inViruses · 2022Article
- Rapid Detection of Strawberry Mild Yellow Edge Virus with a Lateral Flow Strip Reverse Transcription Recombinase Polymerase Amplification Assay.Current microbiology · 2022Article
- Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay.PLoS neglected tropical diseases · 2021Article
- Intestinal Schistosomiasis and Giardiasis Co-Infection in Sub-Saharan Africa: Can a One Health Approach Improve Control of Each Waterborne Parasite Simultaneously?Tropical medicine and infectious disease · 2020Review
- Application of a recombinase polymerase amplification (RPA) assay and pilot field testing for Giardia duodenalis at Lake Albert, Uganda.Parasites & vectors · 2020Article
- Methionyl-tRNA synthetase inhibitor has potent in vivo activity in a novel Giardia lamblia luciferase murine infection model.The Journal of antimicrobial chemotherapy · 2020Article
- Rapid isothermal duplex real-time recombinase polymerase amplification (RPA) assay for the diagnosis of equine piroplasmosis.Scientific reports · 2020Article
- DNA-Detection Based Diagnostics forJournal of parasitology research · 2020Review
- Rapid and accurate detection of carbapenem-resistance gene by isothermal amplification inBurns & trauma · 2020Article
- Multiplex Detection of Nucleic Acids Using Recombinase Polymerase Amplification and a Molecular Colorimetric 7-Segment Display.ACS omega · 2019Article
- Sensitive and rapid detection ofPeerJ · 2019Article
- Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.Scientific reports · 2018Article
- Detection of Babesia gibsoni in dogs by combining recombinase polymerase amplification (RPA) with lateral flow (LF) dipstick.Parasitology research · 2018Article
- Toward the 2020 goal of soil-transmitted helminthiasis control and elimination.PLoS neglected tropical diseases · 2018Article
- Molecular diagnosis of protozoan parasites by Recombinase Polymerase Amplification.Acta tropica · 2018Article
- Recombinase polymerase amplification: Basics, applications and recent advances.Trends in analytical chemistry : TRAC · 2018Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.