ArticleNucleic acids research2015
Comparative functional characterization of the CSR-1 22G-RNA pathway in Caenorhabditis nematodes.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Decoding Argonaute specificity: insights fromRNA (New York, N.Y.) · 2026Review
- piRNAs safeguard splicing and RNA fidelity during heat shock.bioRxiv : the preprint server for biology · 2025Article
- Parental S-adenosylmethionine diet defines offspring immune response via histone H3K4me3 complex and Endoplasmic Reticulum UPR.Cell communication and signaling : CCS · 2025Article
- Lineage-resolved analysis of embryonic gene expression evolution inScience (New York, N.Y.) · 2025Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- Temperature-dependent Small RNA Expression Depends on Wild Genetic Backgrounds of Caenorhabditis briggsae.Molecular biology and evolution · 2022Article
- A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing.Cell reports · 2022Article
- Review
- Small RNA pathways in the nematode Ascaris in the absence of piRNAs.Nature communications · 2022Article
- Article
- Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility.Nucleic acids research · 2021Article
- Small-RNA-mediated transgenerational silencing of histone genes impairs fertility in piRNA mutants.Nature cell biology · 2020Article
- Predicting gene essentiality inComputational and structural biotechnology journal · 2020Article
- Multigenerational Regulation of theAnnual review of genetics · 2019Review
- Molecular evolution across developmental time reveals rapid divergence in early embryogenesis.Evolution letters · 2019Article
- Function and Evolution of Nematode RNAi Pathways.Non-coding RNA · 2019Review
- Article
- Early experiences mediate distinct adult gene expression and reproductive programs in Caenorhabditis elegans.PLoS genetics · 2018Article
- ELLI-1, a novel germline protein, modulates RNAi activity and P-granule accumulation in Caenorhabditis elegans.PLoS genetics · 2017Article
- CeNDR, the Caenorhabditis elegans natural diversity resource.Nucleic acids research · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a champion of small RNA research for two decades, Caenorhabditis elegans has revealed the essential Argonaute CSR-1 to play key nuclear roles in modulating chromatin, chromosome segregation and germline gene expression via 22G-small RNAs. Despite CSR-1 being preserved among diverse nematodes, the conservation and divergence in function of the targets of small RNA pathways remains poorly resolved. Here we apply comparative functional genomic analysis between C. elegans and Caenorhabditis briggsae to characterize the CSR-1 pathway, its targets and their evolution. C. briggsae CSR-1-associated small RNAs that we identified by immunoprecipitation-small RNA sequencing overlap with 22G-RNAs depleted in cbr-csr-1 RNAi-treated worms. By comparing 22G-RNAs and target genes between species, we defined a set of CSR-1 target genes with conserved germline expression, enrichment in operons and more slowly evolving coding sequences than other genes, along with a small group of evolutionarily labile targets. We demonstrate that the association of CSR-1 with chromatin is preserved, and show that depletion of cbr-csr-1 leads to chromosome segregation defects and embryonic lethality. This first comparative characterization of a small RNA pathway in Caenorhabditis establishes a conserved nuclear role for CSR-1 and highlights its key role in germline gene regulation across multiple animal species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.