ArticleProceedings of the National Academy of Sciences of the United States of America1989
Reciprocal inhibition of binding between interleukin 3 and granulocyte-macrophage colony-stimulating factor to human eosinophils.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 130 citations in OpenAlex.
- Dual Role of GM-CSF as a Pro-Inflammatory and a Regulatory Cytokine: Implications for Immune Therapy.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2015Review
- Human airway eosinophils exhibit preferential reduction in STAT signaling capacity and increased CISH expression.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Eosinophils induce airway smooth muscle cell proliferation.Journal of clinical immunology · 2013Article
- The role of granulocyte colony-stimulating factor in the neutrophilia observed in the fetal inflammatory response syndrome.Journal of perinatal medicine · 2011Article
- Familial eosinophilia maps to the cytokine gene cluster on human chromosomal region 5q31-q33.American journal of human genetics · 1998Article
- The direct effect of interferon-gamma on human eosinophilic leukemia cell lines: the induction of interleukin-5 mRNA and the presence of an interferon-gamma receptor.Inflammation · 1996Article
- Identification of receptor-binding domains on human interleukin 5 and design of an interleukin 5-derived receptor antagonist.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Transforming growth factor beta abrogates the effects of hematopoietins on eosinophils and induces their apoptosis.The Journal of experimental medicine · 1994Article
- The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Acute side effects of homologous interleukin-3 in rhesus monkeys.The American journal of pathology · 1993Article
- CD69 is expressed by human eosinophils activated in vivo in asthma and in vitro by cytokines.Immunology · 1993Article
- Interleukin 3 stimulates proliferation and triggers endothelial-leukocyte adhesion molecule 1 gene activation of human endothelial cells.The Journal of clinical investigation · 1993Article
- A human interleukin 3 analog with increased biological and binding activities.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Interleukin-3-induced up-regulation of CR3 expression on human eosinophils is inhibited by dexamethasone.Immunology · 1992Article
- Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3.The Journal of clinical investigation · 1992Article
- Cloning of the low-affinity murine granulocyte-macrophage colony-stimulating factor receptor and reconstitution of a high-affinity receptor complex.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Cytokine-induced (interleukins-3, -6 and -8 and tumour necrosis factor-beta) activation and deactivation of human neutrophils.Immunology · 1992Article
- Eosinophils: a review.Veterinary research communications · 1992Review
- Identification of the second subunit of the murine interleukin-5 receptor: interleukin-3 receptor-like protein, AIC2B is a component of the high affinity interleukin-5 receptor.The EMBO journal · 1991Article
- Recombinant interferon-gamma inhibits the expression of IL-4 receptors on human lymphocytes.Clinical and experimental immunology · 1991Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
125I-labeled recombinant human interleukin 3 (IL-3) bound, at 4 degrees C, to a single class of high-affinity receptors on human eosinophils with an apparent dissociation constant (Kd) of 470 pM, but it did not bind to human neutrophils. 125I-labeled recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) also bound to a single class of high-affinity receptors on eosinophils with an apparent Kd of 44 pM and on neutrophils with an apparent Kd of 70 pM. These binding characteristics were consistent with the biological activities of IL-3 and GM-CSF on eosinophils and with the lack of stimulation of neutrophil function by IL-3. Specificity studies under conditions shown to prevent receptor internalization showed that the binding of 125I-labeled IL-3 to eosinophils was partially inhibited by GM-CSF but not by other cytokines. Reciprocal experiments with 125I-labeled GM-CSF showed that IL-3 but not other cytokines partially inhibited binding to eosinophils. In contrast, the binding of 125I-labeled GM-CSF to neutrophils was not inhibited by IL-3 or other cytokines tested. Quantitative inhibition binding experiments on eosinophils showed that the reciprocal inhibition between IL-3 and GM-CSF was not complete up to a concentration of heterologous ligand of 100 nM. These results show that (i) IL-3 binds to eosinophils but not neutrophils and (ii) IL-3 and GM-CSF specifically interact on the surface of eosinophils, providing a possible mechanism for the overlapping activities of IL-3 and GM-CSF on these cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.